PXD025150 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | LC-MS/MS analysis of pancreatic cancer plasma |
Description | Pancreatic cancer (PC) is an aggressive cancer with a high mortality rate, necessitating the de-velopment of effective diagnostic, prognostic and predictive biomarkers for disease manage-ment. Aberrantly fucosylated proteins in PC are considered a valuable resource of clinically useful biomarkers. The main objective of the present study was to identify novel plasma glyco-biomarkers of PC using the iTRAQ quantitative proteomics approach coupled with Aleuria au-rantia lectin (AAL)-based glycopeptide enrichment and isotope-coded glycosylation site-specific tagging, with a view to analyzing the glycoproteome profiles of plasma samples from patients with non-metastatic and metastatic PC and gallstones (GS). As a result, 22 glycopeptides with significantly elevated levels in plasma samples of PC were identified. Fucosylated SERPINA1 (fuco-SERPINA1) was selected for further validation in 121 plasma samples (50 GS and 71 PC) using an AAL-based reverse lectin ELISA technique developed in-house. Our analyses revealed significantly higher plasma levels of fuco-SERPINA1 in PC than GS subjects (310.7 ng/ml v.s. 153.6 ng/ml, p = 0.0114). Elevated fuco-SERPINA1 levels were associated with higher TNM stage (p = 0.024) and poorer prognosis for overall survival (log-rank test, p = 0.0083). The increased plasma fuco-SERPINA1 levels support the utility of this protein as a novel prognosticator for PC. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_00:15:09.147.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chia-Chun Wu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue; acetylated residue; monohydroxylated residue; deaminated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-04-01 06:47:20 | ID requested | |
⏵ 1 | 2022-02-17 00:15:10 | announced | |
Publication List
Wu CC, Lu YT, Yeh TS, Chan YH, Dash S, Yu JS, Identification of Fucosylated SERPINA1 as a Novel Plasma Marker for Pancreatic Cancer Using Lectin Affinity Capture Coupled with iTRAQ-Based Quantitative Glycoproteomics. Int J Mol Sci, 22(11):(2021) [pubmed] |
Keyword List
submitter keyword: pancreatic cancer, plasma, glycobiomarker, AAL, iTRAQ-based quantitative proteome, reverse lectin-based ELISA, fucosylated SERPINA1 |
Contact List
Jau-Song Yu |
contact affiliation | Molecular Medicine Research Center, Chang Gung University, Taoyuan, Taiwan. |
contact email | yusong@mail.cgu.edu.tw |
lab head | |
Chia-Chun Wu |
contact affiliation | Molecular Medicine Research Center, Chang Gung University |
contact email | wuchiachun9@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025150
- Label: PRIDE project
- Name: LC-MS/MS analysis of pancreatic cancer plasma