PXD025147

PXD025147 is an original dataset announced via ProteomeXchange.

Title	A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation
Description	The RDM15 genomic sequence was fused in frame to the 3xFLAG tag and 3xMYC tag, and the fused sequences were inserted into the pCambia1305 backbone and were transformed into Arabidopsis plants, respectively. A 5-g quantity of flower tissue was harvested from transgenic plants and ground in liquid nitrogen. The proteins were extracted in 25 mL of lysis buffer [50 mM Tris (pH 7.5), 150 mM NaCl, 5 mM MgCl2, 10% glycerol, 0.1% Nonidet P-40, 0.5 mM DTT, 1 mM PMSF, and 250 ul of plant cell protease inhibitor (Sigma)]. After the proteins were extracted, anti-Flag M2 (Sigma; F3165) or anti- Myc (Sigma; M5546) was added and incubated at 4 °C for 2–3 h. The resins were then washed with the lysis buffer at least five times. The protein samples were sent to the Core Facility for Proteomics at the Shanghai Center for Plant Stress Biology (PSC) for affinity purification and mass spectrometry.
HostingRepository	iProX
AnnounceDate	2021-04-01
AnnouncementXML	Submission_2023-02-19_23:33:10.373.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhaobo Lang
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-04-01 05:38:03	ID requested
1	2021-04-01 05:38:29	announced
⏵ 2	2023-02-19 23:33:11	announced	2023-02-20: Update publication information.

Niu Q, Song Z, Tang K, Chen L, Wang L, Ban T, Guo Z, Kim C, Zhang H, Duan CG, Zhang H, Zhu JK, Du J, Lang Z, A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation. Nat Commun, 12(1):3367(2021) [pubmed]

submitter keyword: IP-MS

Zhaobo Lang
contact affiliation	Chinese Academy of Sciences
contact email	zblang@psc.ac.cn
lab head
Zhaobo Lang
contact affiliation	Chinese Academy of Sciences
contact email	zblang@psc.ac.cn
dataset submitter

iProX dataset URI