PXD025126

PXD025126 is an original dataset announced via ProteomeXchange.

Title	Secretome and Proteome of WT and CFTR-KO pancreatic duct epithelial cells
Description	Loss of CFTR function in the pancreatic duct leads to dysregulated luminal pH causing premature activation of digestive enzymes and tissue necrosis. Drastic alterations in pancreatic tissue architecture and cellular composition changes the microenvironment of the islets. Given that CFTR is expressed in the pancreatic ducts, we hypothesized that loss of functional CFTR impacts islet function by modifying the ductal secretome. To this end, we developed a long-term in vitro pancreatic duct epithelial cell culture system and polarized both WT and CFTR-KO (CF) ferret duct epithelial cells. We profiled the apical and basolateral secretome, and the cellular proteome of both WT and CF duct epithelium using quantitative mass spectrometry. Bioinformatic analysis of differentially secreted proteins mapped to their cognate receptors provided a list of putative paracrine interactions that affect islet function. Signaling pathways and upstream regulators that alter the secretome and cellular proteome profile were computationally mined to characterize disease causing mechanisms. In this study, we provide a proteomic roadmap of perturbed autocrine and paracrine signals from the CF pancreatic duct.
HostingRepository	PRIDE
AnnounceDate	2021-05-27
AnnouncementXML	Submission_2021-05-27_06:33:49.215.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025126
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pavana Rotti
SpeciesList	scientific name: Mustela putorius furo; NCBI TaxID: 9669;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-31 06:35:06	ID requested
⏵ 1	2021-05-27 06:33:50	announced

Rotti PG, Evans IA, Zhang Y, Liang B, Cunicelli N, O'Malley Y, Norris AW, Uc A, Engelhardt JF, Lack of CFTR alters the ferret pancreatic ductal epithelial secretome and cellular proteome: Implications for exocrine/endocrine signaling. J Cyst Fibros, 21(1):172-180(2022) [pubmed]

submitter keyword: Cystic Fibrosis, Cystic Fibrosis Related Diabetes, pancreas, ductal cells, secretome, proteome

John F Engelhardt
contact affiliation	Department of Anatomy and Cell Biology, University of Iowa
contact email	john-engelhardt@uiowa.edu
lab head
Pavana Rotti
contact affiliation	University of Iowa
contact email	pavanagururaj-rotti@uiowa.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD025126

PRIDE project URI

• PRIDE
  1. PXD025126
     1. Label: PRIDE project
     2. Name: Secretome and Proteome of WT and CFTR-KO pancreatic duct epithelial cells