PXD025085 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic differences between Drosophila melanogaster female reproductive tract tissue and fluid and their postmating responses |
Description | Fertility depends on the progression of complex and coordinated postmating processes within the extracellular luminal environment of the female reproductive tract (FRT). To achieve a more comprehensive level of knowledge regarding female-derived proteins available to interact with the ejaculate, we utilized semiquantitative mass spectrometry-based proteomics to study the composition of the FRT tissue and, separately, the luminal fluid, before and after mating in Drosophila melanogaster. Our approach leveraged whole-fly isotopic labelling to delineate between female proteins and those transferred from males in the ejaculate. The dynamic mating-induced proteomic changes in the extracellular FRT luminal fluid further informs our understanding of secretory mechanisms of the FRT and serves as a foundation for establishing the roles of ejaculate-female interactions in fertility. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-07 |
AnnouncementXML | Submission_2024-10-07_13:38:40.883.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD025085 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Caitlin McDonough-Goldstein |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | 6x(13)C; 6x(13)C; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-29 12:21:20 | ID requested | |
⏵ 1 | 2024-10-07 13:38:41 | announced | |
Publication List
Keyword List
submitter keyword: Drosophila, reproductoin, ejaculate-female interaction |
Contact List
Steve Dorus |
contact affiliation | Syracuse University Center for Reproductive Evolution |
contact email | sdours@syr.edu |
lab head | |
Caitlin McDonough-Goldstein |
contact affiliation | Syracuse University |
contact email | mcdonouce@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD025085
- Label: PRIDE project
- Name: Proteomic differences between Drosophila melanogaster female reproductive tract tissue and fluid and their postmating responses