PXD025085

PXD025085 is an original dataset announced via ProteomeXchange.

Title	Proteomic differences between Drosophila melanogaster female reproductive tract tissue and fluid and their postmating responses
Description	Fertility depends on the progression of complex and coordinated postmating processes within the extracellular luminal environment of the female reproductive tract (FRT). To achieve a more comprehensive level of knowledge regarding female-derived proteins available to interact with the ejaculate, we utilized semiquantitative mass spectrometry-based proteomics to study the composition of the FRT tissue and, separately, the luminal fluid, before and after mating in Drosophila melanogaster. Our approach leveraged whole-fly isotopic labelling to delineate between female proteins and those transferred from males in the ejaculate. The dynamic mating-induced proteomic changes in the extracellular FRT luminal fluid further informs our understanding of secretory mechanisms of the FRT and serves as a foundation for establishing the roles of ejaculate-female interactions in fertility.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:38:40.883.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025085
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Caitlin McDonough-Goldstein
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	6x(13)C; 6x(13)C; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 12:21:20	ID requested
⏵ 1	2024-10-07 13:38:41	announced

10.6019/PXD025085;

submitter keyword: Drosophila, reproductoin, ejaculate-female interaction

Steve Dorus
contact affiliation	Syracuse University Center for Reproductive Evolution
contact email	sdours@syr.edu
lab head
Caitlin McDonough-Goldstein
contact affiliation	Syracuse University
contact email	mcdonouce@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD025085

PRIDE project URI

• PRIDE
  1. PXD025085
     1. Label: PRIDE project
     2. Name: Proteomic differences between Drosophila melanogaster female reproductive tract tissue and fluid and their postmating responses