PXD025079

PXD025079 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of exosomes isolated from human umbilical cord serum by mass spectrometry and preliminary study of their biological activities in cultured hepatocellular carcinoma cell lines
Description	Exosomes are membranous extracellular vesicles 50–100 nm in size and are involved in cellular communication via the delivery of proteins, lipids, and RNAs. Emerging evidence shows that exosomes play a critical role in cancer. A recent study has revealed that maternal and umbilical cord serum-derived exosomes may enhance endothelial cell proliferation and migration. However, the role of exosomes isolated from the human umbilical cord in cancer development has not been investigated. To explore the potential differences in the composition and function of proteins from umbilical cord blood exosomes and maternal serum exosomes, we conducted a proteomic analysis of exosomes by mass spectrometry and bioinformatics analysis. We used the CCK-8 assay and flow cytometry to study the biological effects of umbilical serum exosomes on hepatoma cells. Our study shows that umbilical cord blood is enriched with proteins involved in ECM-receptor interactions, which may be closely related to cell metastasis and proliferation. Our findings indicate that exosomes derived from human umbilical serum can suppress the viability of hepatoma cells and may induce apoptosis of hepatoma cells. This evidence suggests that umbilical cord serum-derived exosomes may be potential leads for the development of biotherapy for liver cancer.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:10:25.610.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donglie Zhu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 12:18:19	ID requested
⏵ 1	2022-02-17 00:10:26	announced

Zhu D, Li W, Fang C, Yin R, Jiang M, Lv X, Chen Y, Proteomic analysis of human umbilical cord serum exosomes using mass spectrometry and preliminary study of their biological activities in liver cancer cell lines. Exp Ther Med, 23(1):44(2022) [pubmed]

submitter keyword: Exosomes

proteomic analysis

human umbilical cord serum

mass spectrometry

hepatocellular carcinoma

Donglie Zhu
contact affiliation	Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi
contact email	954023642@qq.com
lab head
Donglie Zhu
contact affiliation	Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi
contact email	954023642@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025079

PRIDE project URI

• PRIDE
  1. PXD025079
     1. Label: PRIDE project
     2. Name: Proteomic analysis of exosomes isolated from human umbilical cord serum by mass spectrometry and preliminary study of their biological activities in cultured hepatocellular carcinoma cell lines