PXD025077

PXD025077 is an original dataset announced via ProteomeXchange.

Title	proteomics characterization of the dynamics of ischemic stroke in mice
Description	TMT-coupled proteomic analysis was conducted on mouse brain cortex tissue from five time-points up to four weeks post-stroke in the DH-MCAO mouse model. Nearly-half of the detected proteome was altered following stroke, with <10% of changes at relatively large scales. Clustering on the changed proteome defined four distinct expression patterns characterized by temporal and quantitative changes in innate and adaptive immune response pathways, and cytoskeletal and neuronal remodeling. Further analysis on a subset of top-hits, which temporally responded to stroke with relatively large and sustained changes, revealed that they were highly correlated to different cortical cytokines, and thereby potential pharmacodynamic biomarker candidates for inflammation-targeting therapies. Closer examination of the top enriched neurophysiologic pathways identified 57 proteins potentially associated with post-stroke recovery. Altogether, our study generated a rich dataset with candidate proteins worthy of further validation as biomarkers and/or therapeutic targets for stroke.
HostingRepository	PRIDE
AnnounceDate	2021-06-10
AnnouncementXML	Submission_2021-06-10_01:01:28.436.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ru Wei
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 12:17:05	ID requested
⏵ 1	2021-06-10 01:01:29	announced

Gu RF, Fang T, Nelson A, Gyoneva S, Gao B, Hedde J, Henry K, Peterson E, Burkly LC, Wei R, Proteomic Characterization of the Dynamics of Ischemic Stroke in Mice. J Proteome Res, 20(7):3689-3700(2021) [pubmed]

submitter keyword: MCAO, stroke biomarkers, TMT proteomics, DH-MCAO,stroke, acute ischemic stroke (AIS)

Ru Wei
contact affiliation	Chemical Biology and Proteomics, Biogen 225 Binney Street, Cambridge, MA 02142 United States of America
contact email	ru.wei@biogen.com
lab head
Ru Wei
contact affiliation	Biogen
contact email	ru.wei@biogen.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025077

PRIDE project URI

• PRIDE
  1. PXD025077
     1. Label: PRIDE project
     2. Name: proteomics characterization of the dynamics of ischemic stroke in mice