PXD025069

PXD025069 is an original dataset announced via ProteomeXchange.

Title	The capacity of isoprene emission largely influences the proteome of Arabidopsis plants both under well-watered and water-stress conditions
Description	Isoprene is a small lipophilic molecule synthetized at chloroplast level and released into the atmosphere. Isoprene-emitting plants are believed to be better protected against abiotic stresses (i.e. temperature, oxidative stress, drought). The mechanism of action of isoprene is still under debate. In this study we compared the physiological responses and proteomic profile of isoprene-emitting (ISPS) and non-emitting (WT and EV) Arabidopsis thaliana plants subjected to a moderate water stress and in control conditions. Our aim was to investigate if isoprene-emitting plants displayed a different proteomic profile which may help explain how isoprene protects plants from stresses. Only ISPS plants were able to maintain the same photosynthesis and fresh weight in water stress and in control condition. To better understand the molecular mechanism underlying isoprene emission, we performed a LC-MS/MS-based proteomic analysis to explore the changes in protein abundance between WT and EV both under control and moderate water stress conditions. Our data suggest that isoprene exerts its protective mechanism at different levels rather than on a single mode, triggering significant changes in chloroplast protein profiles, but also playing important roles in modulating signaling and hormone pathways and even membrane trafficking.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-19_22:18:24.779.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milena Marsoni
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 06:48:42	ID requested
⏵ 1	2022-05-19 22:18:25	announced

Mancini I, Domingo G, Bracale M, Loreto F, Pollastri S, Isoprene Emission Influences the Proteomic Profile of Arabidopsis Plants under Well-Watered and Drought-Stress Conditions. Int J Mol Sci, 23(7):(2022) [pubmed]

submitter keyword: Isoprene, Arabidopsis thaliana, proteomics, water stress, defense

Milena Marsoni
contact affiliation	Department of Biotechnology and Life Sciences University of Insubria via H. Dunant, 3 21100 Varese Italy
contact email	milena.marsoni@uninsubria.it
lab head
Milena Marsoni
contact affiliation	University of Insubria
contact email	milena.marsoni@uninsubria.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025069
     1. Label: PRIDE project
     2. Name: The capacity of isoprene emission largely influences the proteome of Arabidopsis plants both under well-watered and water-stress conditions