PXD025064

PXD025064 is an original dataset announced via ProteomeXchange.

Title	A cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1
Description	The TRAPP complexes are nucleotide exchange factors that have essential roles in membrane traffic and autophagy. TRAPPII activates Rab11, and TRAPPIII activates Rab1, with the two complexes sharing a core of small subunits that affect nucleotide exchange, but being distinguished by specific large subunits that are essential for activity in vivo. Crystal structures of core subunits have revealed the mechanism of Rab activation, but how the core and the large subunits assemble to form the complexes was unknown. We report a cryo-EM structure of the entire TRAPPIII complex from Drosophila. The TRAPPIII-specific subunits TRAPPC8 and TRAPPC11 hold the catalytic core like a pair of tongs, with TRAPPC12 and TRAPPC13 positioned at the joint between them. TRAPPC2 and TRAPPC2L link the core to the two large arms, with the interfaces containing residues affected by disease-causing mutations. The TRAPPC8 arm is positioned such that it would contact Rab1 that is bound to the core, indicating how the arm could determine the specificity of the complex. A lower resolution structure of TRAPPII shows a similar architecture, and suggests that the TRAPP complexes evolved from a single ur-TRAPP.
HostingRepository	PRIDE
AnnounceDate	2021-06-03
AnnouncementXML	Submission_2021-06-03_08:29:58.324.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mark Skehel
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-29 04:42:17	ID requested
⏵ 1	2021-06-03 08:29:59	announced

Galindo A, Planelles-Herrero VJ, Degliesposti G, Munro S, Cryo-EM structure of metazoan TRAPPIII, the multi-subunit complex that activates the GTPase Rab1. EMBO J, 40(12):e107608(2021) [pubmed]

submitter keyword: Exchange factor / Rab1 / autophagy / membrane traffic / Golgi

Sean Munro
contact affiliation	MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH United Kingdom
contact email	sean@mrc-lmb.cam.ac.uk
lab head
Mark Skehel
contact affiliation	MRC LMB
contact email	mskehel@mrc-lmb.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/06/PXD025064

PRIDE project URI

• PRIDE
  1. PXD025064
     1. Label: PRIDE project
     2. Name: A cryo-EM structure of metazoan TRAPPIII, the multisubunit complex that activates the GTPase Rab1