PXD025028

PXD025028 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis SKD1 Proteom LC-MSMS
Description	We investigated the heat-dependent in-vivo interactome of Arabidopsis thaliana SKD1 using a transgenic 35S::GFP-SKD1 line. Potential interactors of GFP-SKD1 were co-immunoprecipitated from cell extracts of untreated or heat-treated rosette leaves and analyzed by mass spectrometry. A line overexpressing free YFP (35S::YFP) was used as a negative control. For each genotype and condition, proteins of three biological replicates were analyzed. An in-solution digest was performed on the beads, and peptides were subjected to liquid chromatography and tandem mass spectrometry (LC-MS/MS, Dr. S. Müller, CECAD/CMMC Proteomics Facility Cologne).
HostingRepository	PRIDE
AnnounceDate	2021-06-23
AnnouncementXML	Submission_2021-06-22_22:59:54.978.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Prerana Wagle
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-26 04:46:43	ID requested
⏵ 1	2021-06-22 22:59:55	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, SKD1, Proteom, Heat stress, LC-MSMS

Martin Hülskamp
contact affiliation	University of Cologne, Botanical Institute
contact email	martin.huelskamp@uni-koeln.de
lab head
Prerana Wagle
contact affiliation	CECAD Research Center
contact email	proteomics-facility@uni-koeln.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025028
     1. Label: PRIDE project
     2. Name: Arabidopsis SKD1 Proteom LC-MSMS