PXD025026

PXD025026 is an original dataset announced via ProteomeXchange.

Title	Goat’s milk exosomes as natural nanoparticles in detection of inflammatory processes by optical imaging
Description	Exosomes are cell-derived nanovesicles with a proven key role in intercellular signaling, inflammation processes and immune response. Due to their natural origin and liposome-like structure, these nanometric vesicles have emerged as novel platforms for therapy and diagnosis. This work presents goat’s milk exosomes as natural nanoparticles applied in the detection of inflammatory processes by optical imaging. Goat’s milk exosomes were isolated and fully characterized by their physicochemical properties, proteomics profile and biochemical impact in healthy mice. For the in vitro and in vivo assessment, these nanoparticles were covalently labeled with sulfo-Cyanine 5 and BODIPY-FL. In vitro studies confirmed the internalization of the nanoprobe as well as no cytotoxicity in macrophages populations (RAW 264.7). The capacity of our probe to localize inflammatory processes was in vivo assessed by optical imaging in a peritonitis mouse model confirmed. These results were also confirmed by the flow cytometry analysis of the peritonitis exudates, supporting the use of these natural nanoparticles in the detection of inflammatory processes.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_03:32:14.279.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Capra hircus (Goat); NCBI TaxID: 9925;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-26 04:13:57	ID requested
1	2022-02-21 09:16:15	announced
⏵ 2	2022-02-22 03:32:15	announced	2022-02-22: Updated project metadata.

Santos-Coquillat A, Gonz, á, lez MI, Clemente-Morag, ó, n A, Gonz, á, lez-Arjona M, Albaladejo-Garc, í, a V, Peinado H, Mu, ñ, oz J, Xim, é, nez Emb, ú, n P, Iba, ñ, ez B, Oliver E, Desco M, Salinas B, Goat Milk Exosomes As Natural Nanoparticles for Detecting Inflammatory Processes By Optical Imaging. Small, 18(6):e2105421(2022) [pubmed]

ProteomeXchange project tag: EPIC-XS

submitter keyword: exosome, optical imaging, inflammation, diagnosis,nanoparticle

Javier Muñoz Peralta
contact affiliation	Proteomics Unit Spanish National Cancer Research Centre (CNIO) Melchor Férnandez Almagro, 3. 28029 Madrid. SPAIN
contact email	jmunozpe@cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD025026

PRIDE project URI

• PRIDE
  1. PXD025026
     1. Label: PRIDE project
     2. Name: Goat’s milk exosomes as natural nanoparticles in detection of inflammatory processes by optical imaging