PXD025025

PXD025025 is an original dataset announced via ProteomeXchange.

Title	Systematic Analysis of Lysine Succinylation in Vero cell infected with small ruminant morbillivirus
Description	Small ruminant morbillivirus (SRMV), formerly named Peste-des-petits ruminanats virus (PPRV), belongs to the genus Morbillivirus genus in the family Paramyxoviridae and leads to a highly contagious disease in small ruminants, especially goats and sheep. Lysine succinylation is a newly identified and conserved modification and plays important roles in host cells response to pathogen infection. Herein, we present the comprehensive analysis of the succinylome of SRMV-infected Vero cell using quantitative proteomics. Overall, we identified 875 succinylated proteins with 2840 succinylation sites. Comparative analysis revealed that 139 down-regulated succinylated proteins with 228 succinylation sites and 38 up-regulated succinylated proteins with 44 succinylation sites were significant succinylated in response to SRMV infection, seven lysine succinylation motifs were identified. Bioinformatics analysis showed that the succinalated proteins mainly participated in in cellular respiration and biosynthetic process. Protein-protein interaction networks of the identified proteins provided further evidence that a variety of ATP synthase subunits and carbon metabolism were modulated by succinylation, the overlapped proteins between succinylation and acetylation are involved in glyoxylate and dicarboxylate metabolism. In summary, this is the first study of the succinylome in SRMV infection, lysine acetylation may have a more important effect than succinylation in PPRV infection. It provides a novel view on investigating the infection mechanism of SRMV.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_07:39:16.101.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xuelian meng
SpeciesList	scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	succinylated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-26 04:13:56	ID requested
⏵ 1	2025-05-06 07:39:16	announced

10.1186/s12917-025-04496-3;

Meng X, Zhu X, Wang X, Zhang R, Zhang Z, Sun Y, Comprehensive analysis of the succinylome in Vero cells infected with peste des petits ruminants virus Nigeria 75/1 vaccine strain. BMC Vet Res, 21(1):45(2025) [pubmed]

submitter keyword: post-translational modification； lysine succinylation； protein-protein interaction； Small ruminant morbillivirus

Xuelian meng
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
contact email	mengxuelian@caas.cn
lab head
xuelian meng
contact affiliation	Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
contact email	mengxuelian@caas.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025025
     1. Label: PRIDE project
     2. Name: Systematic Analysis of Lysine Succinylation in Vero cell infected with small ruminant morbillivirus