PXD025024

PXD025024 is an original dataset announced via ProteomeXchange.

Title	Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins
Description	The modification of adenosine to inosine at the wobble position (I34) of tRNA anticodons is an abundant and essential feature of eukaryotic tRNAs. The expansion of inosine-containing tRNAs in eukaryotes followed the transformation of the homodimeric bacterial enzyme TadA, which generates I34 in tRNAArg and tRNALeu, into the heterodimeric eukaryotic enzyme ADAT, which modifies up to eight different tRNAs. The emergence of ADAT and its larger set of substrates, strongly influenced the tRNA composition and codon usage of eukaryotic genomes. However, the selective advantages that drove the expansion of I34-tRNAs remain unknown. Here we investigate the functional relevance of I34-tRNAs in human cells and show that a full complement of these tRNAs is necessary for the translation of low-complexity protein domains enriched in amino acids cognate for I34-tRNAs. The coding sequences for these domains require codons translated by I34-tRNAs, in detriment of synonymous codons that use other tRNAs. I34-tRNA-dependent low-complexity proteins are enriched in functional categories related to cell adhesion, and depletion in I34-tRNAs leads to cellular phenotypes consistent with these roles. We show that the distribution of these low-complexity proteins mirrors the distribution of I34-tRNAs in the phylogenetic tree.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:09:27.563.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Laura Villarreal
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-26 04:13:56	ID requested
⏵ 1	2022-02-17 00:09:28	announced

Torres AG, Rodr, í, guez-Escrib, à M, Marcet-Houben M, Santos Vieira HG, Camacho N, Catena H, Murillo Recio M, Rafels-Ybern À, Reina O, Torres FM, Pardo-Saganta A, Gabald, ó, n T, Novoa EM, Ribas de Pouplana L, Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins. Nucleic Acids Res, 49(12):7011-7034(2021) [pubmed]

submitter keyword: Transfer RNA

Inosine

low-complexity proteins

mucin-like domain

cell adhesion

Lluis Ribas de Pouplana
contact affiliation	Aging $ Metabolism, Gene Translation Laboratory, Institute for Research in Biomedicine, The Barcelona Institute of Science and Technology, Barcelona, Catalonia, Spain.
contact email	lluis.ribas@irbbarcelona.org
lab head
Laura Villarreal
contact affiliation	Research Officer at Mass Spectrometry and Proteomics Core Facility
contact email	laura.villarreal@irbbarcelona.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD025024

PRIDE project URI

• PRIDE
  1. PXD025024
     1. Label: PRIDE project
     2. Name: Human tRNAs with inosine 34 are essential to efficiently translate eukarya-specific low-complexity proteins