PXD025022

PXD025022 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of the cellular effects of AHR activation by microbial tryptophan catabolites in endotoxin-activated human macrophages
Description	Sensing of microbial tryptophan catabolites by the aryl hydrocarbon receptor (AhR) plays a pivotal role in host-microbiome homeostasis by modulating the host immune response. Thereby the involved cellular processes triggered by the metabolites are largely unknown. We analyzed proteomic changes in macrophages trough 24h after treatment with the tryptophan metabolites indole-3-acetic acid (I3AA) or indole-3-aldehyde (IAld), as well as the prototypic AhR-ligand Benzo(a)pyrene (BaP) in the absence and presence of LPS to identify affected processes and pathways.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_07:19:12.552.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025022
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Henning Großkopf
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-26 00:11:30	ID requested
⏵ 1	2021-11-03 07:19:14	announced

Walter K, Grosskopf H, Karkossa I, von Bergen M, Schubert K, Proteomic Characterization of the Cellular Effects of AhR Activation by Microbial Tryptophan Catabolites in Endotoxin-Activated Human Macrophages. Int J Environ Res Public Health, 18(19):(2021) [pubmed]

submitter keyword: AhR, human macrophages, Tryptophan catabolites, Benzo[a]pyrene, Indole-3-aldehyde, Indole-3-acetic acide, Lipopolysaccharides, metabolism, proteomics, LC-MS/MS, TMT

Martin von Bergen
contact affiliation	Department of Molecular Systems Biology; Helmholtz Center for Environmental Research - UFZ, Leipzig, Germany
contact email	martin.vonbergen@ufz.de
lab head
Henning Großkopf
contact affiliation	Molecular Systems Biology Helmholtz Centre for Environmental Research GmbH - UFZ Leipzig, Germany
contact email	henning.grosskopf@ufz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD025022

PRIDE project URI

• PRIDE
  1. PXD025022
     1. Label: PRIDE project
     2. Name: Proteomic characterization of the cellular effects of AHR activation by microbial tryptophan catabolites in endotoxin-activated human macrophages