PXD025013

PXD025013 is an original dataset announced via ProteomeXchange.

Title	Within-host adaptive phenotypic responses of bovine Staphylococcus aureus
Description	The Gram-positive bacterium S. aureus is one of the most common causes of contagious bovine mastitis. A predominant S. aureus clone persists within its host over a long period. In the present study, we compared the surface proteome of two isolates, collected from a naturally infected dairy cow with chronic, subclinical mastitis: an initial isolate (IN) and a host-adapted strain (HA). The isolates were grown under iron- and oxygen-limiting conditions in RPMI 1640 medium. Bacterial cells were isolated at the early stationary phase and used for preparation of surface-associated proteins by applying a surface-shaving and a surface-biotinylation approach. Proteins were identified by gelfree (surface-shaving approach) and GeLC-MS/MS (biotinylation approach) analyses.
HostingRepository	PRIDE
AnnounceDate	2021-08-14
AnnouncementXML	Submission_2021-08-14_06:23:42.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Kucklick
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	biotinylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 10:13:57	ID requested
⏵ 1	2021-08-14 06:23:42	announced

Dataset with its publication pending

submitter keyword: Staphylococcus aureus, cell surface proteome, host adaption, biotinylaton, surface shaving, chronic bovine mastitis

Susanne Engelmann
contact affiliation	Microbial Proteomics, Helmholtz Centre for Infection Research, Braunschweig, Germany; intituite of Microbiology, Technische Universität Braunschweig, Braunschweig, Germany
contact email	Susanne.Engelmann@helmholtz-hzi.de
lab head
Martin Kucklick
contact affiliation	TU Braunschweig Institut für Mikrobiologie Spielmannstr. 7 Germany
contact email	m.kucklick@tu-braunschweig.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD025013

PRIDE project URI

• PRIDE
  1. PXD025013
     1. Label: PRIDE project
     2. Name: Within-host adaptive phenotypic responses of bovine Staphylococcus aureus