PXD025004

PXD025004 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in lifespan extension C4PR_LIV
Description	Dietary restriction (DR) has been shown to increase lifespan in organisms ranging from yeast to mammals. This suggests that the underlying mechanisms may be evolutionarily conserved. Indeed, upstream signalling pathways, such as TOR, are strongly linked to DR-induced longevity in various organisms. However, the downstream effector proteins that ultimately mediate lifespan extension are less clear. To shed light on this, we used a proteomic approach on budding yeast. Our reasoning was that analysis of proteome-wide changes in response to DR might enable the identification of proteins that mediate its physiological effects, including lifespan extension. Of over 2500 proteins we identified by liquid chromatography-mass spectrometry, 183 were significantly altered in expression by at least 3-fold in response to DR. Most of these proteins were mitochondrial and/or had clear links to respiration and metabolism. Indeed, direct analysis of oxygen consumption confirmed that mitochondrial respiration was increased several-fold in response to DR. In addition, several key proteins involved in mating, including Ste2 and Ste6, were downregulated by DR. Consistent with this, shmoo formation in response to α-factor pheromone was reduced by DR, thus confirming the inhibitory effect of DR on yeast mating. Finally, we found that Hsp26, a member of the conserved small heat shock protein (sHSP) family, was upregulated by DR and that overexpression of Hsp26 extends yeast replicative lifespan. As overexpression of sHSPs in C. elegans and Drosophila has previously been shown to extend lifespan, our data on yeast Hsp26 suggest that sHSPs may be universally conserved effectors of longevity.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:03:30.607.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD025004
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Brownridge
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 08:08:48	ID requested
⏵ 1	2022-02-17 00:03:31	announced

Campion R, Bloxam L, Burrow K, Brownridge PJ, Pentland DR, Thomas P, Gourlay CW, Eyers CE, Barclay JW, Morgan A, Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in replicative lifespan extension. Biochem J, 478(24):4153-4167(2021) [pubmed]

submitter keyword: Saccharomyces cerevisiae, calorie restriction, aging, chaperone, mitochondria

Alan Morgan
contact affiliation	Institute of Systems, Molecular and Integrative Biology University of Liverpool, Crown Street, P.O. Box 147, Liverpool, L69 3BX, UK.
contact email	Amorgan@liverpool.ac.uk
lab head
Philip Brownridge
contact affiliation	University of Liverpool
contact email	philipjb@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD025004
     1. Label: PRIDE project
     2. Name: Proteomic analysis of dietary restriction in yeast reveals a role for Hsp26 in lifespan extension C4PR_LIV