PXD024995 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Revealing the human cancer-associated mucinome |
Description | Mucin domains are densely O-glycosylated modular protein domains found in a wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are key players in a host of human diseases, especially cancer, but the scope of the mucinome remains poorly defined. Recently, we characterized a bacterial mucinase, StcE, and demonstrated that an inactive point mutant retains binding selectivity for mucins. In this work, we leveraged inactive StcE to selectively enrich and identify mucins from complex samples like cell lysate and crude ovarian cancer patient ascites fluid. Our enrichment strategy was further aided by an algorithm to assign confidence to mucin-domain glycoprotein identifications. This mucinomics platform facilitated detection of hundreds of glycopeptides from mucin domains and highly overlapping populations of mucin-domain glycoproteins from ovarian cancer patients. Ultimately, we demonstrate our mucinomics approach can reveal key molecular signatures of cancer from in vitro and ex vivo sources. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-30 |
AnnouncementXML | Submission_2022-06-29_18:49:04.727.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Stacy Malaker |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-25 03:28:28 | ID requested | |
⏵ 1 | 2022-06-29 18:49:05 | announced | |
Publication List
Malaker SA, Riley NM, Shon DJ, Pedram K, Krishnan V, Dorigo O, Bertozzi CR, Revealing the human mucinome. Nat Commun, 13(1):3542(2022) [pubmed] |
Keyword List
submitter keyword: O-glycosylation, mucin, mucinase, mucinome, mass spectrometry, proteomics, glycoproteomics, cancer |
Contact List
Stacy Malaker |
contact affiliation | Yale University, Department of Chemistry |
contact email | stacy.malaker@yale.edu |
lab head | |
Stacy Malaker |
contact affiliation | Yale University |
contact email | stacy.malaker@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD024995 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024995
- Label: PRIDE project
- Name: Revealing the human cancer-associated mucinome