PXD024995

PXD024995 is an original dataset announced via ProteomeXchange.

Title	Revealing the human cancer-associated mucinome
Description	Mucin domains are densely O-glycosylated modular protein domains found in a wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are key players in a host of human diseases, especially cancer, but the scope of the mucinome remains poorly defined. Recently, we characterized a bacterial mucinase, StcE, and demonstrated that an inactive point mutant retains binding selectivity for mucins. In this work, we leveraged inactive StcE to selectively enrich and identify mucins from complex samples like cell lysate and crude ovarian cancer patient ascites fluid. Our enrichment strategy was further aided by an algorithm to assign confidence to mucin-domain glycoprotein identifications. This mucinomics platform facilitated detection of hundreds of glycopeptides from mucin domains and highly overlapping populations of mucin-domain glycoproteins from ovarian cancer patients. Ultimately, we demonstrate our mucinomics approach can reveal key molecular signatures of cancer from in vitro and ex vivo sources.
HostingRepository	PRIDE
AnnounceDate	2022-06-30
AnnouncementXML	Submission_2022-06-29_18:49:04.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stacy Malaker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 03:28:28	ID requested
⏵ 1	2022-06-29 18:49:05	announced

Malaker SA, Riley NM, Shon DJ, Pedram K, Krishnan V, Dorigo O, Bertozzi CR, Revealing the human mucinome. Nat Commun, 13(1):3542(2022) [pubmed]

submitter keyword: O-glycosylation, mucin, mucinase, mucinome, mass spectrometry, proteomics, glycoproteomics, cancer

Stacy Malaker
contact affiliation	Yale University, Department of Chemistry
contact email	stacy.malaker@yale.edu
lab head
Stacy Malaker
contact affiliation	Yale University
contact email	stacy.malaker@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD024995

PRIDE project URI

• PRIDE
  1. PXD024995
     1. Label: PRIDE project
     2. Name: Revealing the human cancer-associated mucinome