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PXD024995

PXD024995 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRevealing the human cancer-associated mucinome
DescriptionMucin domains are densely O-glycosylated modular protein domains found in a wide variety of cell surface and secreted proteins. Mucin-domain glycoproteins are key players in a host of human diseases, especially cancer, but the scope of the mucinome remains poorly defined. Recently, we characterized a bacterial mucinase, StcE, and demonstrated that an inactive point mutant retains binding selectivity for mucins. In this work, we leveraged inactive StcE to selectively enrich and identify mucins from complex samples like cell lysate and crude ovarian cancer patient ascites fluid. Our enrichment strategy was further aided by an algorithm to assign confidence to mucin-domain glycoprotein identifications. This mucinomics platform facilitated detection of hundreds of glycopeptides from mucin domains and highly overlapping populations of mucin-domain glycoproteins from ovarian cancer patients. Ultimately, we demonstrate our mucinomics approach can reveal key molecular signatures of cancer from in vitro and ex vivo sources.
HostingRepositoryPRIDE
AnnounceDate2022-06-30
AnnouncementXMLSubmission_2022-06-29_18:49:04.727.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterStacy Malaker
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-25 03:28:28ID requested
12022-06-29 18:49:05announced
Publication List
Malaker SA, Riley NM, Shon DJ, Pedram K, Krishnan V, Dorigo O, Bertozzi CR, Revealing the human mucinome. Nat Commun, 13(1):3542(2022) [pubmed]
Keyword List
submitter keyword: O-glycosylation, mucin, mucinase, mucinome, mass spectrometry, proteomics, glycoproteomics, cancer
Contact List
Stacy Malaker
contact affiliationYale University, Department of Chemistry
contact emailstacy.malaker@yale.edu
lab head
Stacy Malaker
contact affiliationYale University
contact emailstacy.malaker@yale.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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