PXD024991

PXD024991 is an original dataset announced via ProteomeXchange.

Title	Identification of DIR1-dependant cellular responses required for systemic acquired resistance in guard cells
Description	After localized invasion by bacterial pathogens, systemic acquired resistance (SAR) is induced in uninfected plant tissues, resulting in enhanced defense against a broad range of pathogens. Although SAR requires mobilization of signaling molecules via the plant vasculature, the specific mechanisms remain elusive. The lipid transfer protein-defective in induced resistance 1-1 (DIR1-1) was identified in Arabidopsis thaliana by screening for mutants that were defective in SAR. Since then, the structure and lipid-binding properties of DIR1 have been determined, showing that its barrel structure can bind two, long-chain fatty-acid molecules. Several SAR mobile signals, including dehydroabietinal (DA), azelaic acid (AzA), and glycerol-3-phosphate (G3P) are dependent on DIR1 for transport to systemic leaves. Closure of stomata, controlled by guard cells, is a local response to bacteria. Here we demonstrate that stomatal response to pathogens is altered in systemic leaves by SAR, and this guard cell SAR defense requires DIR1. Using a multi-omics approach, we have determined potential SAR signaling mechanisms specific for guard cells in systemic leaves by profiling metabolite, lipid, and protein differences between guard cells in wild type and dir1-1 mutant during SAR. We identified two 18C fatty actyls and two 16C wax esters as putative SAR-related molecules dependent on DIR1. Proteins and metabolites related to amino acid biosynthesis and response to stimulus were altered in guard cells of dir1-1 compared to wild type. Identification of guard cell-specific SAR-related molecules will lead to new avenues of genetic modifications/molecular breeding for disease resistant plants.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_00:11:08.515.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lisa David
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 03:18:10	ID requested
⏵ 1	2022-02-17 00:11:12	announced

David L, Kang J, Nicklay J, Dufresne C, Chen S, Identification of DIR1-Dependant Cellular Responses in Guard Cell Systemic Acquired Resistance. Front Mol Biosci, 8():746523(2021) [pubmed]

submitter keyword: guard cells, proteomics, lipidomics, metabolomics, Arabidopsis thaliana

Sixue Chen
contact affiliation	Department of Biology University of Florida
contact email	schen@ufl.edu
lab head
Lisa David
contact affiliation	University of Florida
contact email	lisaidavid@ufl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024991
     1. Label: PRIDE project
     2. Name: Identification of DIR1-dependant cellular responses required for systemic acquired resistance in guard cells