PXD024978

PXD024978 is an original dataset announced via ProteomeXchange.

Title	Human TRIM72 localizes on the microsome
Description	Membrane repair machinery is a unique process to maintain cell integrity. In the early step of repair process, TRIM72 facilitates the patch membranes to damaged sites for resealing membrane and protects cells from acute membrane injury. The TRIM72 is an E3 ubiquitin ligase belonging to tripartite motif (TRIM) superfamily. Not only the repair machinery, the TRIM72 is also known as targeting membrane proteins such as insulin substrate receptor-1 (IRS-1) and insulin receptor (IR), so membrane binding events would be essential for TRIM72 to play a role. We found that the human TRIM72 localizes on the microsomal vesicles with very strong affinity. By the mass spectrometric gene ontology analysis, it has been confirmed the microsomal fractions were originated from the junctional and the exocytic vesicles. Moreover, identified proteins are classified to the cell adhesion and cell adhesion molecule binding. These results suggest that the cellular activity of TRIM72 would be related to vesicular proteins which engage to cell adhesion and furthermore, it should be mediated after membrane trans-localization of TRIM72.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_05:52:44.133.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sehwan Jang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-25 02:18:27	ID requested
⏵ 1	2024-01-26 05:52:45	announced

10.1038/s41594-023-01111-7;

Park SH, Han J, Jeong BC, Song JH, Jang SH, Jeong H, Kim BH, Ko YG, Park ZY, Lee KE, Hyun J, Song HK, Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane. Nat Struct Mol Biol, 30(11):1695-1706(2023) [pubmed]

submitter keyword: microsome, TRIM72,human

Hyun Kyu Song
contact affiliation	Department of Life Sciences, Korea University, Seoul 02841, Korea
contact email	hksong@korea.ac.kr
lab head
Sehwan Jang
contact affiliation	School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea
contact email	jsh7122@naver.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD024978

PRIDE project URI

• PRIDE
  1. PXD024978
     1. Label: PRIDE project
     2. Name: Human TRIM72 localizes on the microsome