PXD024964 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | CRISPR-Cas9-mediated ALMS1 knockout inhibits TGF-β signalling and epithelial-mesenchyme transition. |
Description | In this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in HeLa cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like focal adhesion or cell-substrate adherens junction. Finally, EMT biomarkers like VIM, DSP, EDIL3 and SNAI1 had the opposite pattern to what would be expected when activating the EMT. In conclusion, seems that the depletion of ALMS1 could be inhibiting the signals transduction through the TGF -β and the routes regulated downstream by it such as the EMT. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:36:16.682.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brais Bea Mascato |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-23 23:36:52 | ID requested | |
1 | 2023-03-10 19:53:32 | announced | |
⏵ 2 | 2023-11-14 08:36:17 | announced | 2023-11-14: Updated project metadata. |
Publication List
Bea-Mascato B, Neira-Goyanes E, Iglesias-Rodr, í, guez A, Valverde D, signalling pathway and downstream processes such as cell migration and adhesion capacity. Front Mol Biosci, 9():992313(2022) [pubmed] |
Keyword List
submitter keyword: epithelial-mesenchyme transition, TGF-β,ALMS1, HeLa, fibrosis, Alström syndrome, LC-ESI-MS/MS |
Contact List
Diana Valverde Pérez |
contact affiliation | University of Vigo |
contact email | dianaval@uvigo.es |
lab head | |
Brais Bea Mascato |
contact affiliation | University of Vigo |
contact email | b.mascat@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD024964 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024964
- Label: PRIDE project
- Name: CRISPR-Cas9-mediated ALMS1 knockout inhibits TGF-β signalling and epithelial-mesenchyme transition.