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PXD024964

PXD024964 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCRISPR-Cas9-mediated ALMS1 knockout inhibits TGF-β signalling and epithelial-mesenchyme transition.
DescriptionIn this project we evaluated the proteomic profiling with TGF-β stimuli at 24h in a CRISPR-Cas9 model for ALMS1 gene in HeLa cells. Proteomic results showed a majority inhibition of downstream regulated pathways by the TGF-β, associating the protein coding genes (PCG) with processes like focal adhesion or cell-substrate adherens junction. Finally, EMT biomarkers like VIM, DSP, EDIL3 and SNAI1 had the opposite pattern to what would be expected when activating the EMT. In conclusion, seems that the depletion of ALMS1 could be inhibiting the signals transduction through the TGF -β and the routes regulated downstream by it such as the EMT.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:36:16.682.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrais Bea Mascato
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-23 23:36:52ID requested
12023-03-10 19:53:32announced
22023-11-14 08:36:17announced2023-11-14: Updated project metadata.
Publication List
Bea-Mascato B, Neira-Goyanes E, Iglesias-Rodr, í, guez A, Valverde D, signalling pathway and downstream processes such as cell migration and adhesion capacity. Front Mol Biosci, 9():992313(2022) [pubmed]
Keyword List
submitter keyword: epithelial-mesenchyme transition, TGF-β,ALMS1, HeLa, fibrosis, Alström syndrome, LC-ESI-MS/MS
Contact List
Diana Valverde Pérez
contact affiliationUniversity of Vigo
contact emaildianaval@uvigo.es
lab head
Brais Bea Mascato
contact affiliationUniversity of Vigo
contact emailb.mascat@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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