PXD024962

PXD024962 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Isolation of RBP47 interactome
Description	Stress granules are dynamic structures, in which components belonging to the shell structure can exchange rapidly while components in the core layer may be less dynamic. The last group of proteins has been suggested to act as a scaffold to which other SG components are recruited. In a previous study, we observed that TSN does not exchange between the cytoplasm and foci upon a fluorescence recovery after photobleaching (FRAP) analysis, suggesting a role as a scaffold protein. Deletion of scaffold-like molecules has been suggest to have a high impact in membraneles organelles composition. Accordingly, and to better understand the role of TSN as a platform for SG components in plants, we characterized the SG proteome by isolation of GFP-tagged RBP47 protein from WT and double tsn1 tsn2 knock-out expressing plants.
HostingRepository	jPOST
AnnounceDate	2021-06-11
AnnouncementXML	Submission_2022-09-18_03:26:38.725.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilio Gutierrez Beltran
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide
Instrument	LTQ Orbitrap

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-23 10:41:56	ID requested
1	2021-06-11 02:34:27	announced
⏵ 2	2022-09-18 03:26:39	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: cytoplasmic membraneless organelles (MLOs), biomolecular condesates, ribonucleoprotein (RNP) complex, RNA-binding protein (RBP), SGs proteome, stress granules (SGs), Tudor Staphylococcal Nuclease (TSN)

submitter keyword: cytoplasmic membraneless organelles (MLOs), biomolecular condesates, ribonucleoprotein (RNP) complex, RNA-binding protein (RBP), SGs proteome, stress granules (SGs), Tudor Staphylococcal Nuclease (TSN)

Contact List

Emilio Gutierrez-Beltran
lab head
Emilio Gutierrez Beltran
contact affiliation	University of Sevilla
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001103/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001103/