PXD024942

PXD024942 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Integrin a5 Associated Complexes Dependent upon Integrin Activation State in Zebrafish
Description	Integrins are a major class of heterodimeric adhesion receptors composed of an a and b subunit that link the extracellular matrix (ECM) and the cytoskeleton across the cell membrane. When activated by either the intracellular (inside-out signaling) or extracellular (outside-in signaling) environment, integrins undergo a conformational change that increases the ligand binding affinity. As the primary receptor for ECM protein fibronectin, Integrin a5 plays a critical role in zebrafish somitogenesis. To better understand integrin activation in this context, we performed co-immunoprecipitation and Mass Spectrometry (MS) based proteomics using FLAG-tagged Integrin a5 alleles that alter it activation state: constitutive active mutant a5GAAKR and inactive ligand binding deficient mutant a5FYLDD, expressed in maternal zygotic a5 mutant (MZa5-/-) embryos. Our data provide an overview of Integrin associated proteins according to the activation state of the Integrin.
HostingRepository	PRIDE
AnnounceDate	2021-05-27
AnnouncementXML	Submission_2021-05-27_13:59:44.680.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Holley
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-23 03:39:18	ID requested
⏵ 1	2021-05-27 13:59:45	announced

Dataset with its publication pending

submitter keyword: Integrin a5, integrin activation, zebrafish, 12 somite stage, extracellular matrix, cell adhesion, LC-MSMS

Scott A. Holley
contact affiliation	Department of Molecular, Cellular and Developmental Biology. Yale University, 260 Whitney Ave, New Haven, CT. 06520, USA
contact email	scott.holley@yale.edu
lab head
Scott Holley
contact affiliation	Yale University
contact email	scott.holley@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024942

PRIDE project URI

• PRIDE
  1. PXD024942
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Integrin a5 Associated Complexes Dependent upon Integrin Activation State in Zebrafish