PXD024933

PXD024933 is an original dataset announced via ProteomeXchange.

Title	Examining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells
Description	Methanogens were recently shown to reduce pyrite (FeS2) generating aqueous iron-sulfide (FeS(aq)) clusters that are likely assimilated as a source of Fe and S. Here, we compare the phenotype of Methanococcus voltae when grown with FeS2 or ferrous iron (Fe(II)) and sulfide (HS-). Differential proteomic analyses showed similar expression of core methanogenesis enzymes, indicating that Fe and S source does not substantively alter the energy metabolism of cells. However, a homolog of the Fe(II) transporter FeoB and its transcriptional regulator DtxR were up-expressed in FeS2 grown cells, indicating that cells sense Fe(II) limitation. Two homologs of IssA, a protein putatively involved in coordinating thioferrate nanoparticles, were also up-expressed in FeS2 grown cells. We interpret these data to indicate that DtxR cannot sense Fe(II) and therefore cannot down-regulate FeoB. We suggest this is due to the transport of Fe(II) complexed with sulfide (FeS(aq)) leading to excess Fe that is sequestered by IssA as a thioferrate-like species. This model provides a framework for the design of targeted experiments aimed at further characterizing Fe acquisition and homeostasis in M. voltae and other methanogens.
HostingRepository	PRIDE
AnnounceDate	2021-07-07
AnnouncementXML	Submission_2021-07-07_13:47:31.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Spietz
SpeciesList	scientific name: Methanococcus voltae A3; NCBI TaxID: 456320;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-23 00:21:28	ID requested
⏵ 1	2021-07-07 13:47:31	announced

Dataset with its publication pending

submitter keyword: methanogen, iron-sulfur cluster, FeS2, pyrite, mackinawite, FeS

Boyd, Eric S.
contact affiliation	Department of Microbiology and Immunology, Montana State University, Bozeman, Montana, 59717
contact email	eric.boyd@montana.edu
lab head
Rachel Spietz
contact affiliation	Montana State University
contact email	rachel.spietz@montana.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD024933

PRIDE project URI

• PRIDE
  1. PXD024933
     1. Label: PRIDE project
     2. Name: Examining pathways of iron and sulfur acquisition, trafficking, deployment, and storage in mineral-grown methanogen cells