PXD024917

PXD024917 is an original dataset announced via ProteomeXchange.

Title	Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants
Description	SureQuant-MHC utilizes a series of isotopologues and internal standard-triggered targeted mass spectrometry to generate an embedded multipoint calibration curve to determine the endogenous pMHC concentrations for a panel of 18 antigen targets. We apply this methiod to profile changes in pMHC expression levels in melanoma cell lines treated with binimetinib, a MEK inhibitr, and traslate the approach to profiling antigen levels in human tumor punch biopsies.
HostingRepository	PRIDE
AnnounceDate	2021-09-21
AnnouncementXML	Submission_2021-09-21_13:00:55.994.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauren Stopfer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 04:37:18	ID requested
⏵ 1	2021-09-21 13:00:56	announced

Stopfer LE, Gajadhar AS, Patel B, Gallien S, Frederick DT, Boland GM, Sullivan RJ, White FM, Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants. Proc Natl Acad Sci U S A, 118(37):(2021) [pubmed]

submitter keyword: Lc-MS/MS, SureQuant, MHC

Forest White
contact affiliation	Department of Biological Engineering
contact email	fwhite@mit.edu
lab head
Lauren Stopfer
contact affiliation	MIT
contact email	lstopfer@mit.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024917

PRIDE project URI

• PRIDE
  1. PXD024917
     1. Label: PRIDE project
     2. Name: Absolute quantification of tumor antigens using embedded MHC-I isotopologue calibrants