PXD024915

PXD024915 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Chemical proteomics identifies a novel off-target protein of small molecule NLRP3 inflammasome inhibitor MCC950
Description	Inhibition of inflammasome and pyroptotic pathways are promising strategies for clinical treatment of autoimmune and inflammatory disorders. MCC950, a potent inhibitor of the NLR-family inflammasome pyrin domain-containing 3 (NLRP3) protein, has shown encouraging results in animal models for a range of conditions; however, little is known about its off-target effects. We have designed, synthesized and applied a novel photoaffinity alkyne-tagged probe for MCC950 which shows direct engagement with NLRP3 and inhibition of inflammasome activation in macrophages. Affinity-based chemical proteomics in live macrophages identified several potential off-targets, and independent cellular thermal proteomic profiling revealed stabilization of these by MCC950. This is the results of the thermal protein profiling.
HostingRepository	PRIDE
AnnounceDate	2021-05-17
AnnouncementXML	Submission_2021-05-16_22:28:11.912.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cassandra Kennedy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 04:37:04	ID requested
⏵ 1	2021-05-16 22:28:12	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Human, THP1, MCC950, LC-MS/MS

submitter keyword: Human, THP1, MCC950, LC-MS/MS

Contact List

Edward W Tate
contact affiliation	Department of Chemistry, Imperial College London, UK
contact email	e.tate@imperial.ac.uk
lab head
Cassandra Kennedy
contact affiliation	Imperial College London
contact email	c.kennedy16@imperial.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024915

PRIDE project URI

Repository Record List

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