PXD024905

PXD024905 is an original dataset announced via ProteomeXchange.

Title	Pull-down assay to identify proteins interacting with the small protein AtpΘ (Norf1) in Synechocystis sp. PCC 6803
Description	Small proteins in prokaryotes are usually defined as polypeptides that are shorter than 80 amino acids. In the model cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803), the existence of several small proteins was verified previously. Among them, Norf1 (renamed to AtpΘ afterwards) is a small protein that is highly accumulated in darkness or stationary phase. To clarify the functions of AtpΘ, co-immunoprecipitation was performed to identify its interaction partners using FLAG-tagged AtpΘ protein as bait and FLAG-tagged sfGFP as negative control in three biological replicates each.
HostingRepository	PRIDE
AnnounceDate	2022-04-14
AnnouncementXML	Submission_2022-04-13_23:51:50.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Maass
SpeciesList	scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 04:36:00	ID requested
⏵ 1	2022-04-13 23:51:51	announced

Song K, Baumgartner D, Hagemann M, Muro-Pastor AM, Maa, ß S, Becher D, Hess WR, ATP synthase to arrest ATP hydrolysis during low-energy conditions in cyanobacteria. Curr Biol, 32(1):136-148.e5(2022) [pubmed]

submitter keyword: protein pull-down

small protein AtpΘ

F0F1 ATP synthase

Synechocystis sp. PCC 6803

Dörte Becher
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Proteomics, Felix-Hausdorff-Str. 8, 17489 Greifswald, Germany
contact email	dbecher@uni-greifswald.de
lab head
Sandra Maass
contact affiliation	University of Greifswald, Department for Microbial Proteomics
contact email	sandra.maass@uni-greifswald.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024905
     1. Label: PRIDE project
     2. Name: Pull-down assay to identify proteins interacting with the small protein AtpΘ (Norf1) in Synechocystis sp. PCC 6803