PXD024892

PXD024892 is an original dataset announced via ProteomeXchange.

Title	Proteomics and Bioinformatics Analysis of Cardiovascular Related Proteins in Offspring Exposed to Gestational Diabetes Mellitus
Description	Introduction Previous studies have demonstrated that exposed to the initial suboptimal intrauterine environment of gestational diabetes mellitus (GDM) may increase risk of cardiovascular disease in adulthood. Methods In order to investigate the underlying mechanisms involved in the increased risk of cardiovascular diseases in the offspring of GDM, we applied a high-throughput proteomics approach to compare the proteomic expression profile of human umbilical vessels of normal and GDM offspring. Results A total of significantly different 100 proteins were identified in umbilical vessels from GDM group compared with normal controls, among which 31 proteins were up-regulated, while 69 proteins were down-regulated. Differentially expressed proteins (DEPs) are validated using Western blotting analysis. The analysis of these differently expressed proteins (DEPs) related diseases and functions results, performed by Ingenuity Pathway Analysis (IPA) software. Based on 'Diseases and Disorders' analysis, 17 proteins (ACTA2, ADAR, CBFB, DDAH1, FBN1, FGA, FGB, FGG, GLS, GSTM1, HBB, PGM3, PPP1R13L, S100A8, SLC12A4, TPP2, VCAN) were described to be associated with Cardiovascular disease, especially in Anemia, Thrombus and Myocardial infarction. Functional analysis indicated that DEPs involved in many cardiovascular functions, especially in 'vasoconstriction of blood vessel' (related DEPs: ACTA2, DDAH1, FBN1, FGA, FGB and FGG). Upstream regulator analyses of DEPs identifies STAT3 as inhibitor of ACTA2, FGA, FGB and FGG. Conclusion The results of this study indicate that intrauterine hyperglycemia is associated with an elevated risk of cardiovascular risk in the offspring.
HostingRepository	iProX
AnnounceDate	2021-03-20
AnnouncementXML	Submission_2023-02-12_23:47:10.045.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haitao Pan
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 00:19:08	ID requested
1	2021-03-22 00:20:14	announced
⏵ 2	2023-02-12 23:47:11	announced	2023-02-13: Update publication information.

Pan HT, Xiong YM, Zhu HD, Shi XL, Yu B, Ding HG, Xu RJ, Ding JL, Zhang T, Zhang J, Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus. Front Cardiovasc Med, 9():1021112(2022) [pubmed]

submitter keyword: Gamete and Embryo-Fetal Origins of Adult Diseases, Gestational Diabetes Mellitus, Offspring, Umbilical Vessel, iTRAQ

Haitao Pan
contact affiliation	Shaoxing Maternity and Child Health Care Hospital
contact email	panhaitao2007@163.com
lab head
Haitao Pan
contact affiliation	Shaoxing Maternity and Child Health Care Hospital
contact email	11218082@zju.edu.cn
dataset submitter

iProX dataset URI