PXD024890

PXD024890 is an original dataset announced via ProteomeXchange.

Title	Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence
Description	Peste des Petits Ruminants (PPR) is a highly infectious disease caused by a virus of the genus Morbillivirus (PPRV), infecting mainly sheep and goat. Susceptibility of host can vary widely with host breed and virus strain. The mechanisms underlying this variability are not well understood. Here, we carried out the first comparative in vitro study on goat peripheral blood mononuclear cells (PBMCs) infected with PPRV strains of different virulence, vaccine strain (N75/1), low- (IC89), and high-virulent strain (MA08). Goat PBMCs were infected by the different strains and stimulated with Concanavalin A (Con A), and proteome changes of these cells were evaluated during the infection. The level of PPRV replication was assessed first by RT-qPCR quantification of viral N mRNA and by labelling the viral N protein inside the cells with specific antibodies and analysed by flow cytometry. The dynamics of PBMC sub-populations were also evaluated by flow cytometry. Our results showed that viral replication is critical for PPRV inhibitory effect on PBMCs proliferation. Highly virulent MA08 strain reached a higher level of replication in ConA-stimulated PBMCs and induced higher mortality compared to other strains. Low-virulent IC89 strain showed the lower replication level and cell proliferative inhibitory capacities. Differences in immune genes expression levels were assessed using RNAseq analysis and protein expression was compared using mass spectrometry. Analysis of these data provided the transcriptional and proteome landscape of PBMCs infected with these strains. The possible association between the transcriptional and proteome landscape and changes in immune cell subpopulations dynamics was explored.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_07:47:39.125.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024890
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Functional Proteomics Platform FPP
SpeciesList	scientific name: Capra hircus; NCBI TaxID: 9925; scientific name: Peste des petits ruminants virus; NCBI TaxID: 2593991;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-22 00:06:14	ID requested
⏵ 1	2021-11-03 07:47:40	announced

Eloiflin RJ, Auray G, Python S, Rodrigues V, Seveno M, Urbach S, El Koulali K, Holzmuller P, Totte P, Libeau G, Bataille A, Summerfield A, Identification of Differential Responses of Goat PBMCs to PPRV Virulence Using a Multi-Omics Approach. Front Immunol, 12():745315(2021) [pubmed]

submitter keyword: Peste des Petits Ruminants Virus, PBMC, Goat, Immune response, Virulence, Proteomics morbillivirus

Arnaud Bataille
contact affiliation	AnimalS health Territories Risks Ecosystems (ASTRE Unit CIRAD), France
contact email	arnaud.bataille@cirad.fr
lab head
Functional Proteomics Platform FPP
contact affiliation	CNRS
contact email	contact@fpp.cnrs.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024890
     1. Label: PRIDE project
     2. Name: Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence