PXD024872

PXD024872 is an original dataset announced via ProteomeXchange.

Title	Global and diGLY enriched proteomes of RanBPM depleted HeLa cells and RanBPM APMS
Description	The human CTLH complex is a newly discovered multi-subunit E3 ligase. At present, only a few of its ubiquitination targets are known. Here, we use proteomic techniques in RanBPM-depleted HeLa cells to identify ubiquitination substrates of the CTLH complex. First, global proteomics determined proteins that were significantly increased in cells depleted of RanBPM. This analysis revealed potential degradation-induced ubiquitination substrates of the complex. Ubiquitination differences using diGLY-enriched proteomics in shRanBPM cells compared with the endogenous RanBPM interactome also revealed candidate ubiquitination targets.
HostingRepository	PRIDE
AnnounceDate	2021-08-16
AnnouncementXML	Submission_2021-08-16_00:07:12.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Maitland
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive Plus; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-19 03:51:37	ID requested
1	2021-08-13 09:01:18	announced
⏵ 2	2021-08-16 00:07:14	announced	2021-08-16: Updated project metadata.

Maitland MER, Kuljanin M, Wang X, Lajoie GA, Schild-Poulter C, Proteomic analysis of ubiquitination substrates reveals a CTLH E3 ligase complex-dependent regulation of glycolysis. FASEB J, 35(9):e21825(2021) [pubmed]

10.1096/FJ.202100664R;

submitter keyword: Human, HeLa, CTLH complex

Caroline Schild-Poulter
contact affiliation	Robarts Research Institute, Department of Biochemistry,Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada, N6A 5B7
contact email	cscild-poulter@robarts.ca
lab head
Matthew Maitland
contact affiliation	University of Western Ontario
contact email	mmaitla2@uwo.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD024872

PRIDE project URI

• PRIDE
  1. PXD024872
     1. Label: PRIDE project
     2. Name: Global and diGLY enriched proteomes of RanBPM depleted HeLa cells and RanBPM APMS