PXD024864

PXD024864 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	ABIN1 is an signal-induced autophagy adaptor counteracting LUBAC-mediated linear ubiquitination
Description	ABIN1 KO RAW264.7 cells and stably reconstituted with 3×FLAG-ABIN1 WT or D485N were subjected to mass spectrometric analysis. Three biological replicates were prepared for each condition. Cells were stimulated for 3 hours with 100 ng/ml LPS. ABIN1 was immunoprecipitated with 30 uL anti-FLAG M2 Magnetic Beads (M8823, Sigma-Aldrich) for 2 h at 4℃. Immunoprecipitated sample was eluted by incubation at 90°C for 5 min in 100 uL PTS buffer. Each sample was split into 3 tubes for protein digestion with Lys-C and trypsin, chymotrypsin, or Glu-C.
HostingRepository	jPOST
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2024-03-12_19:36:51.003.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; deamidated L-glutamine; deamidated L-asparagine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-19 02:39:26	ID requested
1	2022-02-16 07:00:04	announced
2	2022-09-18 03:48:26	announced	2022-09-18: Updated FTP location.
⏵ 3	2024-03-12 19:36:51	announced	2024-03-13: Updated PubMed.

Publication List

Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K, B activation by recognizing linear ubiquitin chains. FEBS Lett, 596(9):1147-1164(2022) [pubmed]

Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K, B activation by recognizing linear ubiquitin chains. FEBS Lett, 596(9):1147-1164(2022) [pubmed]

Keyword List

submitter keyword: ABIN1, NF-kB, MyDDsome, autophagy, linear ubiquitination

submitter keyword: ABIN1, NF-kB, MyDDsome, autophagy, linear ubiquitination

Contact List

Kazuhiro Iwai
lab head
Koshi Imami
contact affiliation	RIKEN
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001110/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001110/