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PXD024864

PXD024864 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleABIN1 is an signal-induced autophagy adaptor counteracting LUBAC-mediated linear ubiquitination
DescriptionABIN1 KO RAW264.7 cells and stably reconstituted with 3×FLAG-ABIN1 WT or D485N were subjected to mass spectrometric analysis. Three biological replicates were prepared for each condition. Cells were stimulated for 3 hours with 100 ng/ml LPS. ABIN1 was immunoprecipitated with 30 uL anti-FLAG M2 Magnetic Beads (M8823, Sigma-Aldrich) for 2 h at 4℃. Immunoprecipitated sample was eluted by incubation at 90°C for 5 min in 100 uL PTS buffer. Each sample was split into 3 tubes for protein digestion with Lys-C and trypsin, chymotrypsin, or Glu-C.
HostingRepositoryjPOST
AnnounceDate2022-02-17
AnnouncementXMLSubmission_2024-03-12_19:36:51.003.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKoshi Imami
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; deamidated L-glutamine; deamidated L-asparagine
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-19 02:39:26ID requested
12022-02-16 07:00:04announced
22022-09-18 03:48:26announced2022-09-18: Updated FTP location.
32024-03-12 19:36:51announced2024-03-13: Updated PubMed.
Publication List
Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K, B activation by recognizing linear ubiquitin chains. FEBS Lett, 596(9):1147-1164(2022) [pubmed]
Keyword List
submitter keyword: ABIN1, NF-kB, MyDDsome, autophagy, linear ubiquitination
Contact List
Kazuhiro Iwai
lab head
Koshi Imami
contact affiliationRIKEN
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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