PXD024864
PXD024864 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | ABIN1 is an signal-induced autophagy adaptor counteracting LUBAC-mediated linear ubiquitination |
Description | ABIN1 KO RAW264.7 cells and stably reconstituted with 3×FLAG-ABIN1 WT or D485N were subjected to mass spectrometric analysis. Three biological replicates were prepared for each condition. Cells were stimulated for 3 hours with 100 ng/ml LPS. ABIN1 was immunoprecipitated with 30 uL anti-FLAG M2 Magnetic Beads (M8823, Sigma-Aldrich) for 2 h at 4℃. Immunoprecipitated sample was eluted by incubation at 90°C for 5 min in 100 uL PTS buffer. Each sample was split into 3 tubes for protein digestion with Lys-C and trypsin, chymotrypsin, or Glu-C. |
HostingRepository | jPOST |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2024-03-12_19:36:51.003.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Koshi Imami |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; deamidated L-glutamine; deamidated L-asparagine |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-19 02:39:26 | ID requested | |
1 | 2022-02-16 07:00:04 | announced | |
2 | 2022-09-18 03:48:26 | announced | 2022-09-18: Updated FTP location. |
⏵ 3 | 2024-03-12 19:36:51 | announced | 2024-03-13: Updated PubMed. |
Publication List
Shinkawa Y, Imami K, Fuseya Y, Sasaki K, Ohmura K, Ishihama Y, Morinobu A, Iwai K, B activation by recognizing linear ubiquitin chains. FEBS Lett, 596(9):1147-1164(2022) [pubmed] |
Keyword List
submitter keyword: ABIN1, NF-kB, MyDDsome, autophagy, linear ubiquitination |
Contact List
Kazuhiro Iwai | |
---|---|
lab head | |
Koshi Imami | |
contact affiliation | RIKEN |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001110/ |