PXD024851
PXD024851 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Display of the Human Mucinome with Defined O-Glycans by Gene Engineered Cells |
Description | Mucins are a large family of heavily O-glycosylated proteins that cover all mucosal surfaces and constitute the major macromolecules in most body fluids. Mucins can form aggregated networks and bundles that serve as a barrier, but also assist in containing, feeding, clearing and replenishing microorganisms. Mucins are primarily defined by their variable tandem repeat (TR) domains that are densely decorated with different O-glycan structures in distinct patterns, and these arguably convey much of the informational content of mucins. However, the O-glycodomains have long evaded detailed structural and functional exploration. Here, we developed a cell-based platform for the display and production of human TR O-glycodomains (~200 amino acids) with tunable structures and patterns of O-glycans using membrane-bound and secreted constructs expressed in glycoengineered HEK293 cells. The expressed mucin TRs revealed surprisingly high fidelity in complete decoration with designed O-glycan structures, which enabled intact mass analysis with the simplest glycoforms. Availability of defined mucin TR O-glycodomains advances experimental studies into the versatile role of mucins at the interface with pathogenic microorganisms and the microbiome, and sparks new strategies for molecular dissection of specific roles of adhesins, glycoside hydrolases, glycopeptidases, viruses and other interactions with mucin TRs as highlighted by examples. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-11 |
AnnouncementXML | Submission_2021-07-19_22:45:44.893.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Sergey Vakhrushev |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Hex-HexNAc |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-03-19 00:00:21 | ID requested | |
1 | 2021-05-11 03:39:51 | announced | |
⏵ 2 | 2021-07-19 22:45:45 | announced | 2021-07-20: Updated publication reference for PubMed record(s): 34210959. |
Publication List
Nason R, B, ü, ll C, Konstantinidi A, Sun L, Ye Z, Halim A, Du W, S, ø, rensen DM, Durbesson F, Furukawa S, Mandel U, Joshi HJ, Dworkin LA, Hansen L, David L, Iverson TM, Bensing BA, Sullam PM, Varki A, Vries E, de Haan CAM, Vincentelli R, Henrissat B, Vakhrushev SY, Clausen H, Narimatsu Y, Display of the human mucinome with defined O-glycans by gene engineered cells. Nat Commun, 12(1):4070(2021) [pubmed] |
Keyword List
submitter keyword: Intact Mass, OrbiTrap, Mucin, Glycosylation |
Contact List
Sergey Vakhrushev | |
---|---|
contact affiliation | Copenhagen Center for Glycomics (CCG), Glyco-MS Group Department of Cellular and Molecular Medicine University of Copenhagen |
contact email | seva@sund.ku.dk |
lab head | |
Sergey Vakhrushev | |
contact affiliation | Department of Cellular and Molecular Medicine |
contact email | seva@sund.ku.dk |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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