PXD024836

PXD024836 is an original dataset announced via ProteomeXchange.

Title	Improving sperm cryopreservation with type III antifreeze proteins: effect on proteomic profiles of Cynomolgus macaque sperm
Description	Antifreeze protein III (AFP III) has been used in the cryopreservation of germ cells in several kinds of animals, but it is not yet known what the specific cryoprotective mechanism of AFP III is, except for reducing the formation of ice crystals during cryopreservation. In order to study the cryoprotective mechanism of AFP III on sperm cryopreservation, the proteomic profile of Cynomolgus macaques sperm were identified after cryopreservation. Compared to fresh sperm, 141 and 32 proteins were differentially identified in Cynomolgus macaques sperm cryopreserved without and with 0.1µg/ml AFP III, respectively. These proteins were mainly involved in mitochondrial production of reactive oxygen species (ROS), glutathione (GSH) synthesis and cell apoptosis. According to the differential proteins, we supposed that AFP Ⅲ mainly reduce sperm lipid, protein and DNA damage, as well as the release of cytochrome c, and thereby reduce sperm apoptosis by modulating the production of ROS in mitochondria. The molecular mechanism that how AFP III acts with sperm proteins and protects cell from cryoinjuries needs further study.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:37:57.933.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bingbing Chen
SpeciesList	scientific name: Cynomolgus macaque cytomegalovirus strain Mauritius; NCBI TaxID: 1690255;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 07:39:50	ID requested
⏵ 1	2022-02-16 23:37:58	announced

Chen B, Wang S, Inglis BM, Ding H, Suo A, Qiu S, Duan Y, Li X, Li S, Sun WQ, Si W, ) Sperm. Front Physiol, 12():719346(2021) [pubmed]

submitter keyword: Cynomolgus macaques

sperm

cryopreservation

antifreeze protein III

proteomic profile

Bingbing Chen
contact affiliation	Institute of Biothermal Science and Technology, School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology
contact email	chenbengbeng@hotmail.com
lab head
Bingbing Chen
contact affiliation	Institute of Biothermal Science and Technology, School of Medical Instruments and Food Engineering, University of Shanghai for Science and Technology
contact email	chenbengbeng@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD024836

PRIDE project URI

• PRIDE
  1. PXD024836
     1. Label: PRIDE project
     2. Name: Improving sperm cryopreservation with type III antifreeze proteins: effect on proteomic profiles of Cynomolgus macaque sperm