PXD024833

PXD024833 is an original dataset announced via ProteomeXchange.

Title	Identification of the interaction proteins of PRSS37 by GFP-IP/MS using testis proteins from PRSS37-EGFP knock-in mice and pAcr-EGFP transgenic mice
Description	To identify the potential interaction partners of PRSS37, three testis lysates of Prss37E/+ mice (PRSS37-EGFP knock-in heterozygous mice) and three testis lysates of pAcr-EGFP transgenic mice were immunoprecipitated using anti-GFP mAb magnetic beads. The GFP-IP products of six samples from two groups were subsequently analyzed by mass spectrometry (MS) to identify differentially immmunoprecipitated proteins (DIPs) in the PRSS37-EGFP group. Proteins detected in pAcr-EGFP group were used as negative controls to exclude the endogenous proteins and polypeptides that interact nonspecifically with the anti-GFP mAb magnetic beads.
HostingRepository	PRIDE
AnnounceDate	2021-05-06
AnnouncementXML	Submission_2021-05-06_06:01:30.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chunling Shen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 07:39:35	ID requested
⏵ 1	2021-05-06 06:01:30	announced

Dataset with its publication pending

submitter keyword: Mouse, Testis, PRSS37, PRSS37-EGFP knock-in mice, pAcr-EGFP transgenic mice, GFP-immunoprecipitation, differentially immunoprecipitated proteins

Zhugang Wang
contact affiliation	Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
contact email	zhugangw@shsmu.edu.cn
lab head
Chunling Shen
contact affiliation	Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
contact email	clshen@139.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/05/PXD024833

PRIDE project URI

• PRIDE
  1. PXD024833
     1. Label: PRIDE project
     2. Name: Identification of the interaction proteins of PRSS37 by GFP-IP/MS using testis proteins from PRSS37-EGFP knock-in mice and pAcr-EGFP transgenic mice