PXD024819

PXD024819 is an original dataset announced via ProteomeXchange.

Title	Investigation of protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator
Description	The aim of this study was to understand how autotrophic (CO2-fixing) bacteria balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment.To this end, the proteome of the model chemolithoautotroph Ralstonia eutropha a.k.a. Cupriavidus necator was studied in different environmental conditions (four limiting substrates, and five different growth rates). Cupriavidus was cultivated in substrate-limited chemostats with fructose, formate, succinate and ammonium limitation to obtain steady state cell samples. The dilution rate/growth rate was increased step-wise from 0.05 to 0.25 1/h in 0.05 steps. Protein quantity was determined by LC-MS, and enzyme utilization was investigated by resource balance analysis modeling.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:48:10.759.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024819
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael Jahn
SpeciesList	scientific name: Cupriavidus necator H16; NCBI TaxID: 381666;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-18 01:23:40	ID requested
⏵ 1	2022-02-16 23:48:11	announced

Jahn M, Crang N, Janasch M, Hober A, Forsstr, ö, m B, Kimler K, Mattausch A, Chen Q, Asplund-Samuelsson J, Hudson EP, . Elife, 10():(2021) [pubmed]

submitter keyword: Cupriavidus necator, Ralstonia eutropha, cellular economy, resource allocation, resource balance, substrate limitation, shotgun proteomics, data-dependent acquisition, openMS, MSGF+

Paul Hudson
contact affiliation	1-Department of protein Science, KTH Royal Institute of Technology, Stockholm, Sweden, 2-Science for Life Laboratory, Stockholm, Sweden
contact email	paul.hudson@scilifelab.se
lab head
Michael Jahn
contact affiliation	Science for Life Laboratory Stockholm
contact email	michael.jahn@scilifelab.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD024819

PRIDE project URI

• PRIDE
  1. PXD024819
     1. Label: PRIDE project
     2. Name: Investigation of protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator