PXD024809

PXD024809 is an original dataset announced via ProteomeXchange.

Title	Immunopeptidomics DIA and DDA Dynamic Range Experiment
Description	The peptide identification sensitivity of the affinity purified immunopeptidome of a JY cell line (Epstein–Barr virus-immortalized B cell lymphoblastoid line, ECACC 94022533, IHW9287) for DDA and DIA experiments was assessed by a linear concentration series. JY cells are homozygous for all HLA-I loci (HLA-A*02:01, HLA-B*07:02, and HLA-C*07:02). The JY HLA-I ligandome was isolated through immunoaffinity purification, as described in Marcu, Bichmann and Kuchenbecker et al 2021.
HostingRepository	PRIDE
AnnounceDate	2025-09-04
AnnouncementXML	Submission_2025-09-03_19:42:13.001.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ana Marcu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 11:22:37	ID requested
⏵ 1	2025-09-03 19:42:13	announced

10.1136/JITC-2025-012083;

submitter keyword: dynamic range, DIA, immunopeptidomics,HLA

Hans-Georg Rammensee
contact affiliation	Institute for Cell Biology Dept. Immunology Auf der Morgenstelle 15, 72076 Tübingen Germany Partner Site of the German Cancer Consortium
contact email	rammensee@uni-tuebingen.de
lab head
Ana Marcu
contact affiliation	University of Tübingen, Interfaculty Institute for Cell Biology, Department of Immunology
contact email	anamarcu.pe@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/09/PXD024809

PRIDE project URI

• PRIDE
  1. PXD024809
     1. Label: PRIDE project
     2. Name: Immunopeptidomics DIA and DDA Dynamic Range Experiment