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PXD024794 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBANP is an essential transcription factor that opens chromatin and activates CpG island regulated genes
DescriptionWhile the majority of RNA polymerase II initiation events in mammalian genomes take place within CpG island (CGI) promoters, our understanding of their regulation remains limited. Here we combine single-molecule footprinting with interaction proteomics to identify BANP as a critical CGI regulator and the long sought-after TF that binds the orphan CGCG element in mouse and human. We show that BANP drives the activity of essential metabolic genes in the mouse genome in pluripotent and terminally differentiated cells. However, BANP binding is strongly repelled by DNA methylation of its motif in vitro and in vivo, which epigenetically restricts most binding to CGIs and accounts for its absence at aberrantly methylated CGIs in cancer cells. Upon binding to an unmethylated motif, BANP opens chromatin and phases nucleosomes. Our results establish Banp as a critical activator and put forth a model whereby CGI promoter activity relies on methylation-sensitive TFs capable of chromatin opening.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterVytautas Iesmantavicius
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-17 08:05:02ID requested
12021-06-16 09:27:17announced
22021-08-12 22:05:22announced2021-08-13: Updated publication reference for PubMed record(s): 34234345.
Publication List
Grand RS, Burger L, Gräwe C, Michael AK, Isbel L, Hess D, Hoerner L, Iesmantavicius V, Durdu S, Pregnolato M, Krebs AR, Smallwood SA, Thomä N, Vermeulen M, Schübeler D, BANP opens chromatin and activates CpG-island-regulated genes. Nature, 596(7870):133-137(2021) [pubmed]
Keyword List
submitter keyword: BANP, CpG island, single-molecule footprinting, TF binding, CGI promoters, PAQMAN
Contact List
Dirk Schübeler
contact affiliationFriedrich Miescher Institute for Biomedical Research, Basel, Switzerland
contact emaildirk.schubeler@fmi.ch
lab head
Vytautas Iesmantavicius
contact affiliationFriedrich Miescher Institute for Biomedical Research
contact emailvytautas.iesmantavicius@fmi.ch
dataset submitter
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