PXD024791

PXD024791 is an original dataset announced via ProteomeXchange.

Title	The C-terminus of the PSMA3 proteasome subunit preferentially traps intrinsically disordered proteins for degradation
Description	Degradation of intrinsically disordered proteins (IDPs) by a non-26S proteasome process does not require proteasomal targeting by polyubiquitin. However, whether and how IDPs are recognized by the non-26S proteasome, including the 20S complex, remains unknown. Analyses of protein interactome datasets revealed that the 20S proteasome subunit, PSMA3, preferentially interacts with many IDPs. Employing in vivo and cell-free experiments, it was found that a 69-amino-acids-long fragment at the C-terminus of PSMA3 is sufficient to bind the disordered protein p21. A recombinant PSMA3 C-terminus 69 fragment is sufficient to interact with many IDPs, and is therefore designated an IDP trapper. A recombinant IDP trapper blocks the degradation of many IDPs in vitro by the 20S proteasome, possibly by competing with the native trapper. In addition, over a third of the PSMA3 trapper-binding proteins have previously been identified as 20S proteasome substrates, and based on published datasets many of the trapper binding proteins are associated with the intracellular proteasomes. The PSMA3-trapped IDPs that are proteasome substrates have the unique features previously recognized as characteristic 20S proteasome substrates in vitro. We propose a model whereby the PSMA3 C-terminal region traps a subset of IDPs to facilitate their proteasomal degradation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:35:54.132.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024791
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yishai Levin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 04:42:35	ID requested
1	2023-03-10 19:41:15	announced
⏵ 2	2023-11-14 08:35:54	announced	2023-11-14: Updated project metadata.

10.6019/PXD024791;

Biran A, Myers N, Steinberger S, Adler J, Riutin M, Broennimann K, Reuven N, Shaul Y, The C-Terminus of the PSMA3 Proteasome Subunit Preferentially Traps Intrinsically Disordered Proteins for Degradation. Cells, 11(20):(2022) [pubmed]

submitter keyword: proteasome, disordered proteins, proteomics

Yosef Shaul
contact affiliation	Weizmann Institute of Science
contact email	yosef.shaul@weizmann.ac.il
lab head
Yishai Levin
contact affiliation	Weizmann Institute of Science, Israel
contact email	yishai.levin@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024791
     1. Label: PRIDE project
     2. Name: The C-terminus of the PSMA3 proteasome subunit preferentially traps intrinsically disordered proteins for degradation