PXD024788

PXD024788 is an original dataset announced via ProteomeXchange.

Title	Multi-level microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles
Description	The anaerobic digestion microbiomes has been puzzling us since the dawn of molecular methods for mixed microbial community analysis. Monitoring of the anaerobic digestion microbiome can either take place via a holistic evaluation of the microbial community through fingerprinting or by targeted monitoring of selected taxa. Here, we compared four different microbial community fingerprinting methods, i.e., amplicon sequencing, metaproteomics, metabolomics and phenotypics, in their ability to reflect the full-scale anaerobic digestion microbiome. The phenotypic fingerprinting reflects a, for anaerobic digestion, novel, single cell-based approach of direct microbial community fingerprinting. Three different digester types, i.e., sludge digesters, digesters treating agro-industrial waste and dry anaerobic digesters reflected different operational parameters. The α-diversity analysis yielded inconsistent results, especially for richness, across the different methods. In contrast, β-diversity analysis resulted in comparable profiles, even when translated into phyla or functions, with clear separation of the three digester types. In-depth analysis of each method's features i.e., operational taxonomic units, metaproteins, metabolites, and phenotypic traits, yielded certain similar features yet, also some clear differences between the different methods, which was related to the complexity of the anaerobic digestion process. In conclusion, phenotypic fingerprinting is a reliable, fast method for holistic monitoring of the anaerobic digestion microbiome, and the complementary identification of key features through other methods could give rise to a direct interpretation of anaerobic digestion process performance.
HostingRepository	PRIDE
AnnounceDate	2021-05-26
AnnouncementXML	Submission_2021-05-26_08:09:52.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Heyer
SpeciesList	scientific name: human gut metagenome; NCBI TaxID: 408170; scientific name: anaerobic digester metagenome; NCBI TaxID: 1263854;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 04:42:17	ID requested
⏵ 1	2021-05-26 08:09:52	announced

Dataset with its publication pending

submitter keyword: 16S rRNA gene amplicon sequencing, anaerobic digestion, flow cytometry, metabolomics, metaproteomics, microbial fingerprinting

Dirk Benndorf
contact affiliation	Otto von Guericke Universität Magdeburg
contact email	benndorf@mpi-magdeburg.mpg.de
lab head
Robert Heyer
contact affiliation	OvGU
contact email	heyer@mpi-magdeburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024788
     1. Label: PRIDE project
     2. Name: Multi-level microbial fingerprinting in anaerobic digestion reveals consistent fingerprinting profiles