PXD024784

PXD024784 is an original dataset announced via ProteomeXchange.

Title	Radiation resistance comparable to natural extremophiles achieved through experimental evolution
Description	Recent human exploitation of high-level sources of ionizing radiation (IR) has generated a corresponding need to understand the effects of IR on living systems. Part of that research has focused on the astonishing capacity of some organisms to survive extraordinary levels of IR exposure. Using experimental evolution, we have generated populations of Escherichia coli with an IR resistance phenotype comparable to the extremophile Deinococcus radiodurans. Every aspect of cell physiology is affected. Cellular isolates exhibit approximately 1,000 base pair changes plus major genomic and proteomic alterations. The extreme IR resistance phenotype, stable without selection for at least 100 generations, has no single molecular origin. Defined and probable contributions include alterations in cellular systems involved in DNA repair, amelioration of reactive oxygen species, Fe metabolism and repair of iron-sulfur centers, DNA packaging, intermediary metabolism, and more. Some contributions to IR resistance are highly dependent on both genomic and environmental context.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:38:33.874.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Minkoff
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Deinococcus radiodurans; NCBI TaxID: 1299;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-17 00:08:40	ID requested
⏵ 1	2022-02-16 23:38:34	announced

Bruckbauer ST, Minkoff BB, Sussman MR, Cox MM, Proteome Damage Inflicted by Ionizing Radiation: Advancing a Theme in the Research of Miroslav Radman. Cells, 10(4):(2021) [pubmed]

submitter keyword: Radiation, E coli, D radiodurans, Oxidation

Michael R Sussman
contact affiliation	Center for Genomic Science Innovation Department of Biochemistry University of Wisconsin-Madison
contact email	msussman@wisc.edu
lab head
Benjamin Minkoff
contact affiliation	University of Wisconsin-Madison
contact email	bminkoff@wisc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024784
     1. Label: PRIDE project
     2. Name: Radiation resistance comparable to natural extremophiles achieved through experimental evolution