PXD024782

PXD024782 is an original dataset announced via ProteomeXchange.

Title	Balanced Input from the tRNA Prenyltransferase MiaA Controls the Stress Resistance and Virulence Potential of Extraintestinal Pathogenic Escherichia coli
Description	In Escherichia coli, the highly conserved enzymes MiaA and MiaB mediate the sequential prenylation and methylthiolation of adenosine-37 within tRNAs that decode UNN codons. We found that MiaA, but not MiaB, is critical to the fitness and virulence of extraintestinal pathogenic E. coli (ExPEC), a major cause of urinary tract and bloodstream infections. Deletion of miaA has pleiotropic effects, attenuating bacterial fitness and virulence within diverse host environments and rendering ExPEC especially sensitive to stressors like nitrogen and oxygen radicals and osmotic shock. We find that stress can stimulate striking changes in miaA expression. To assess how changing MiaA levels affect the pathogen proteome, we used MS to analyze the proteins express by the reference ExPEC isolate UTI89 and derivatives that either lack or overexpress MiaA.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_06:44:41.821.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Connor Beebout
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 23:49:38	ID requested
⏵ 1	2022-02-14 06:44:42	announced

Dataset with its publication pending

submitter keyword: tRNA modification, translation, fidelity, MiaA, MiaB, sepsis, urinary tract infection, proteome, translational regulation, stress response, frameshifting, bacterial fitness, uropathogenic, ExPEC, Extraintestinal pathogenic Escherichia coli

Matthew A Mulvey
contact affiliation	Division of Microbiology and Immunology, Pathology Department, University of Utah School of Medicine, Salt Lake City, Utah
contact email	mulvey@path.utah.edu
lab head
Connor Beebout
contact affiliation	Vanderbilt University
contact email	connor.j.beebout@vanderbilt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD024782

PRIDE project URI

• PRIDE
  1. PXD024782
     1. Label: PRIDE project
     2. Name: Balanced Input from the tRNA Prenyltransferase MiaA Controls the Stress Resistance and Virulence Potential of Extraintestinal Pathogenic Escherichia coli