PXD024770

PXD024770 is an original dataset announced via ProteomeXchange.

Title	2DG-induced protein glycosylation alterations in colorectal cancer cells CX1 using differential glycosite profiling
Description	A new strategy to compare cellular glycoproteomes, thereby identifying membrane proteins with altered glycan structures and the concerned glycosites. The workflow consists of membrane proteins digestion followed by lectin-based isolation of glycopeptides and their fractionation. Since alterations in the glycan part of a glycopeptide causes mass alterations, analytical size exclusion chromatography is applied to detect these mass shifts. A combination of N-glycosidase treatment with nanoUPLC coupled Exploris-Orbitrap mass spectrometry identifies the altered glycoproteins and the respective glycosites. The methodology was established using the human colon cancer cell line CX1 which was treated with 2-deoxy-glucose - a modulator of N-glycosylation.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:19:50.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Malwina Michalak
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; (18)O label at both C-terminal oxygens; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 05:33:50	ID requested
⏵ 1	2022-02-16 23:19:51	announced

Michalak M, Kalteis MS, Ahadova A, Kloor M, Kriegsmann M, Kriegsmann K, Warnken U, Helm D, Kopitz J, Differential Glycosite Profiling-A Versatile Method to Compare Membrane Glycoproteomes. Molecules, 26(12):(2021) [pubmed]

submitter keyword: Glycoproteomics, 2-deoxy-glucose, glycopeptide, glycosite, colorectal cancer

Jürgen Kopitz
contact affiliation	Department of Applied Tumor Biology, Institute of Pathology, University Hospital Heidelberg, Germany
contact email	juergen.kopitz@med.uni-heidelberg.de
lab head
Malwina Michalak
contact affiliation	Institute of Pathology, University Hospital Heidelberg
contact email	malwina.michalak@med.uni-heidelberg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024770
     1. Label: PRIDE project
     2. Name: 2DG-induced protein glycosylation alterations in colorectal cancer cells CX1 using differential glycosite profiling