PXD024769

PXD024769 is an original dataset announced via ProteomeXchange.

Title	Cytosine methylation of tRNA in Plasmodium falciparum disrupts translational homeostasis to prime for sexual commitment
Description	Malaria parasites have evolved a well-adjusted developmental program to progress through the complex life cycle in the human and mosquito host. Here we identify cytosine methylation of tRNAAsp (GTC) as being critical to maintain stable protein synthesis under cellular stress. Using conditional knock out of a member of the DNA methyltransferases family called Pf-DNMT2, RNA bisulfite sequencing demonstrates the selective cytosine methylation of this enzyme of tRNAAsp (GTC) at position C38. Although no growth defect on parasite asexual proliferation was observed, Pf-DNMT2 KO parasites show a striking increase in their sexual commitment and an increased sensitivity to Dihydroartemisinin. In mutant parasites we observe a dramatic decrease of tRNAAsp (GTC) when compared to non-stressed parasites and mass spectrometry analysis shows the selective downregulation of proteins with GAC codon bias. Here we identify an epitranscriptomic mechanism that safeguards protein translation and homeostasis of sexual commitment during cellular stress in malaria parasites
HostingRepository	PRIDE
AnnounceDate	2022-02-13
AnnouncementXML	Submission_2022-02-13_03:09:03.967.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	AMEYA SINHA
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 04:58:11	ID requested
⏵ 1	2022-02-13 03:09:04	announced

Hammam E, Sinha A, Baumgarten S, Nardella F, Liang J, Miled S, Bonhomme F, Erdmann D, Arcangioli B, Arimondo PB, Dedon P, Preiser P, Scherf A, Malaria Parasite Stress Tolerance Is Regulated by DNMT2-Mediated tRNA Cytosine Methylation. mBio, 12(6):e0255821(2021) [pubmed]

submitter keyword: DMT2, tRNA, Plasmodium, Epitranscriptomics

Peter C Dedon
contact affiliation	Biological Engineering, CEHS, MIT
contact email	pcdedon@mit.edu
lab head
AMEYA SINHA
contact affiliation	NTU
contact email	ameya.sinha@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024769
     1. Label: PRIDE project
     2. Name: Cytosine methylation of tRNA in Plasmodium falciparum disrupts translational homeostasis to prime for sexual commitment