⮝ Full datasets listing

PXD024763

PXD024763 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular characterisation of the SL1 snRNP and other factors involved in spliced leader trans-splicing in the nematode Caenorhabditis elegans using immunoprecipitation and LC-MS/MS.
DescriptionSpliced leader trans-splicing is an essential RNA processing step that is required for the formation of mRNA in many eukaryotes, including C. elegans. However, the factors involved in this reaction are not well known. Here we perform a molecular analysis of key components in this reaction by immunoprecipitation of GFP-tagged SNA-1 and SNA-3 proteins from C. elegans embryonic extracts treated with/without RNAseA/T1 followed by the identification of associated proteins using LC-MS/MS and label-free quantification. As control, embryonic extract from wild type N2 animals were also subjected to the same treatment. Note file names (e.g. PE906_SNA1_RNase_IP_anti-GFP_beads) indicate the name of the C. elegans line (PE906_), the name of the protein tagged with GFP (SNA1) and whether samples were treated with RNases A and T1 (RNase), and for raw files whether immunoprecipitation was done with anti-GFP nanobody coupled agarose beads (IP_anti_GFP_beads), or control agarose beads (IP_control_beads), respectively. Note that SNA-3 protein is only identified by its Uniprot identifier Q9GYR5.
HostingRepositoryPRIDE
AnnounceDate2022-06-24
AnnouncementXMLSubmission_2022-06-24_01:54:56.784.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBerndt Muller
SpeciesList scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-16 04:57:43ID requested
12022-06-24 01:54:57announced
Publication List
Fasimoye RY, Spencer REB, Soto-Martin E, Eijlers P, Elmassoudi H, Brivio S, Mangana C, Sabele V, Rechtorikova R, Wenzel M, Connolly B, Pettitt J, M, ΓΌ, ller B, A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex. Nucleic Acids Res, 50(13):7591-7607(2022) [pubmed]
Keyword List
submitter keyword: RNA processing, small nuclear ribonucleoproteins, nematode
Contact List
Berndt Muller
contact affiliationUniversity of Aberdeen School of Medicine, Medical Sciences and Nutrition Institute of Medical Sciences Foresterhill Aberdeen AB25 2ZD Scotland, United Kingdom
contact emailb.mueller@abdn.ac.uk
lab head
Berndt Muller
contact affiliationUniversity of Aberdeen
contact emailb.mueller@abdn.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD024763
PRIDE project URI
Repository Record List
[ + ]