PXD024763

PXD024763 is an original dataset announced via ProteomeXchange.

Title	Molecular characterisation of the SL1 snRNP and other factors involved in spliced leader trans-splicing in the nematode Caenorhabditis elegans using immunoprecipitation and LC-MS/MS.
Description	Spliced leader trans-splicing is an essential RNA processing step that is required for the formation of mRNA in many eukaryotes, including C. elegans. However, the factors involved in this reaction are not well known. Here we perform a molecular analysis of key components in this reaction by immunoprecipitation of GFP-tagged SNA-1 and SNA-3 proteins from C. elegans embryonic extracts treated with/without RNAseA/T1 followed by the identification of associated proteins using LC-MS/MS and label-free quantification. As control, embryonic extract from wild type N2 animals were also subjected to the same treatment. Note file names (e.g. PE906_SNA1_RNase_IP_anti-GFP_beads) indicate the name of the C. elegans line (PE906_), the name of the protein tagged with GFP (SNA1) and whether samples were treated with RNases A and T1 (RNase), and for raw files whether immunoprecipitation was done with anti-GFP nanobody coupled agarose beads (IP_anti_GFP_beads), or control agarose beads (IP_control_beads), respectively. Note that SNA-3 protein is only identified by its Uniprot identifier Q9GYR5.
HostingRepository	PRIDE
AnnounceDate	2022-06-24
AnnouncementXML	Submission_2022-06-24_01:54:56.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Berndt Muller
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 04:57:43	ID requested
⏵ 1	2022-06-24 01:54:57	announced

Fasimoye RY, Spencer REB, Soto-Martin E, Eijlers P, Elmassoudi H, Brivio S, Mangana C, Sabele V, Rechtorikova R, Wenzel M, Connolly B, Pettitt J, M, ü, ller B, A novel, essential trans-splicing protein connects the nematode SL1 snRNP to the CBC-ARS2 complex. Nucleic Acids Res, 50(13):7591-7607(2022) [pubmed]

submitter keyword: RNA processing, small nuclear ribonucleoproteins, nematode

Berndt Muller
contact affiliation	University of Aberdeen School of Medicine, Medical Sciences and Nutrition Institute of Medical Sciences Foresterhill Aberdeen AB25 2ZD Scotland, United Kingdom
contact email	b.mueller@abdn.ac.uk
lab head
Berndt Muller
contact affiliation	University of Aberdeen
contact email	b.mueller@abdn.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024763
     1. Label: PRIDE project
     2. Name: Molecular characterisation of the SL1 snRNP and other factors involved in spliced leader trans-splicing in the nematode Caenorhabditis elegans using immunoprecipitation and LC-MS/MS.