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PXD024759

PXD024759 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman chorionic villus proteome
DescriptionWe evaluated the effect of the different solubilization techniques on the reproducibility and width of the chorionic proteome characterized using bottom-up proteomics: SDS-based solubilization coupled with in-gel protein digestion after applying self-named 1DE gel-concentration procedure (SDS-PAGE without fractionating in the resolving gel for SDS removal) and in-solution protein digestion of urea-thiourea extracts. These results indicated that 1DE-concentration procedure coupled with in-gel digestion, LC-MS/MS and combinational usage of different bioinformatics tools provides in-depth analysis of a single protein band, including reliable identification and quantification of low-abundant proteins, e.g. FUCA1, PSG7.
HostingRepositoryPRIDE
AnnounceDate2022-06-21
AnnouncementXMLSubmission_2022-06-21_06:20:20.791.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOlesya Larina
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-16 04:57:28ID requested
12022-06-21 06:20:21announced
Publication List
Shkrigunov T, Pogodin P, Zgoda V, Larina O, Kisrieva Y, Klimenko M, Latyshkevich O, Klimenko P, Lisitsa A, Petushkova N, Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi. Curr Issues Mol Biol, 44(5):2069-2088(2022) [pubmed]
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
submitter keyword: Human, Chorionic villus, Trophoblast, LC-MS/MS
Contact List
Petushkova Natalia
contact affiliationInstitute of Biomedical Chemistry laboratory of microsomal oxidation
contact emailcyp450@mail.ru
lab head
Olesya Larina
contact affiliationdr
contact emaillarina.ibmc@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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