PXD024759 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human chorionic villus proteome |
Description | We evaluated the effect of the different solubilization techniques on the reproducibility and width of the chorionic proteome characterized using bottom-up proteomics: SDS-based solubilization coupled with in-gel protein digestion after applying self-named 1DE gel-concentration procedure (SDS-PAGE without fractionating in the resolving gel for SDS removal) and in-solution protein digestion of urea-thiourea extracts. These results indicated that 1DE-concentration procedure coupled with in-gel digestion, LC-MS/MS and combinational usage of different bioinformatics tools provides in-depth analysis of a single protein band, including reliable identification and quantification of low-abundant proteins, e.g. FUCA1, PSG7. |
HostingRepository | PRIDE |
AnnounceDate | 2022-06-21 |
AnnouncementXML | Submission_2022-06-21_06:20:20.791.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Olesya Larina |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-03-16 04:57:28 | ID requested | |
⏵ 1 | 2022-06-21 06:20:21 | announced | |
Publication List
Shkrigunov T, Pogodin P, Zgoda V, Larina O, Kisrieva Y, Klimenko M, Latyshkevich O, Klimenko P, Lisitsa A, Petushkova N, Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi. Curr Issues Mol Biol, 44(5):2069-2088(2022) [pubmed] |
Keyword List
ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project |
submitter keyword: Human, Chorionic villus, Trophoblast, LC-MS/MS |
Contact List
Petushkova Natalia |
contact affiliation | Institute of Biomedical Chemistry laboratory of microsomal oxidation |
contact email | cyp450@mail.ru |
lab head | |
Olesya Larina |
contact affiliation | dr |
contact email | larina.ibmc@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD024759
- Label: PRIDE project
- Name: Human chorionic villus proteome