PXD024759

PXD024759 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Human chorionic villus proteome
Description	We evaluated the effect of the different solubilization techniques on the reproducibility and width of the chorionic proteome characterized using bottom-up proteomics: SDS-based solubilization coupled with in-gel protein digestion after applying self-named 1DE gel-concentration procedure (SDS-PAGE without fractionating in the resolving gel for SDS removal) and in-solution protein digestion of urea-thiourea extracts. These results indicated that 1DE-concentration procedure coupled with in-gel digestion, LC-MS/MS and combinational usage of different bioinformatics tools provides in-depth analysis of a single protein band, including reliable identification and quantification of low-abundant proteins, e.g. FUCA1, PSG7.
HostingRepository	PRIDE
AnnounceDate	2022-06-21
AnnouncementXML	Submission_2022-06-21_06:20:20.791.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Olesya Larina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-16 04:57:28	ID requested
⏵ 1	2022-06-21 06:20:21	announced

Publication List

Shkrigunov T, Pogodin P, Zgoda V, Larina O, Kisrieva Y, Klimenko M, Latyshkevich O, Klimenko P, Lisitsa A, Petushkova N, Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi. Curr Issues Mol Biol, 44(5):2069-2088(2022) [pubmed]

Shkrigunov T, Pogodin P, Zgoda V, Larina O, Kisrieva Y, Klimenko M, Latyshkevich O, Klimenko P, Lisitsa A, Petushkova N, Protocol for Increasing the Sensitivity of MS-Based Protein Detection in Human Chorionic Villi. Curr Issues Mol Biol, 44(5):2069-2088(2022) [pubmed]

Keyword List

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project
submitter keyword: Human, Chorionic villus, Trophoblast, LC-MS/MS

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

submitter keyword: Human, Chorionic villus, Trophoblast, LC-MS/MS

Contact List

Petushkova Natalia
contact affiliation	Institute of Biomedical Chemistry laboratory of microsomal oxidation
contact email	cyp450@mail.ru
lab head
Olesya Larina
contact affiliation	dr
contact email	larina.ibmc@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD024759
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD024759

PRIDE project URI

Repository Record List

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