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PXD024756

PXD024756 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRegulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases
DescriptionHistone H3 lysine 9 dimethylation (H3K9me2) is a highly conserved silencing epigenetic mark. Chromatin marked with H3K9me2 forms large domains in mammalian cells and overlaps well with lamina-associated domains and the B compartment defined by Hi-C. However, the role of H3K9me2 in 3-dimensional (3D) genome organization remains unclear. We investigated genome-wide H3K9me2 distribution, transcriptome, and 3D genome organization in mouse embryonic stem cells following the inhibition or depletion of five H3K9 methyltransferases (MTases): G9a, GLP, SETDB1, SUV39H1, and SUV39H2. H3K9me2 was regulated by all five MTases; however, H3K9me2 and transcription in the A and B compartments were regulated by different MTases. H3K9me2 in A compartments was primarily regulated by G9a/GLP and SETDB1, while H3K9me2 in the B compartments was regulated by all five MTases. Furthermore, decreased H3K9me2 correlated with changes to the more active compartmental state that accompanied transcriptional activation.
HostingRepositoryPRIDE
AnnounceDate2021-08-05
AnnouncementXMLSubmission_2021-08-05_02:24:24.544.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD024756
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterTakehiro Suzuki
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; acetylated residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-03-16 04:57:13ID requested
12021-08-05 02:24:25announced
Publication List
Fukuda K, Shimura C, Miura H, Tanigawa A, Suzuki T, Dohmae N, Hiratani I, Shinkai Y, Regulation of mammalian 3D genome organization and histone H3K9 dimethylation by H3K9 methyltransferases. Commun Biol, 4(1):571(2021) [pubmed]
Keyword List
submitter keyword: Histone H3 methylation
Contact List
Naoshi Dohmae
contact affiliationBiomolecular characterization unit, Center for Sustainable Resource Science, RIKEN
contact emaildohmae@riken.jp
lab head
Takehiro Suzuki
contact affiliationRIKEN Center for Sustainable Resource Science
contact emailtakehirosuzuki@riken.jp
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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