PXD024743

PXD024743 is an original dataset announced via ProteomeXchange.

Title	Parallel G-quadruplexes recruit the HSV-1 transcription factor ICP4 to promote viral transcription in herpes virus-infected human cells
Description	G-quadruplexes (G4s) are four-stranded nucleic acid structures abundant at gene promoters. They can adopt several distinctive conformations. G4s have been shown to form in the herpes simplex virus-1 (HSV-1) genome during its viral cycle. Here by cross-linking/pull-down assay we identified ICP4, the major HSV-1 transcription factor, as the protein that most efficiently interacts with viral G4s during infection. ICP4 specific and direct binding and unfolding of parallel G4s, including those present in HSV-1 immediate early gene promoters, induced transcription in vitro and in infected cells. This mechanism was also exploited by ICP4 to promote its own transcription. Proximity ligation assay allowed visualization of G4-protein interaction at the single selected G4 in cells. G4 ligands inhibited ICP4 binding to G4s. Our results indicate the existence of a well-defined G4-viral protein network that regulates the productive HSV-1 cycle. They also point to G4s as elements that recruit transcription factors to activate transcription in cells.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:18:59.169.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilaria Frasson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Human alphaherpesvirus 1; NCBI TaxID: 10298;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Xevo G2-S QTof

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-15 08:15:39	ID requested
⏵ 1	2022-02-16 23:19:00	announced

Frasson I, Sold, à P, Nadai M, Lago S, Richter SN, Parallel G-quadruplexes recruit the HSV-1 transcription factor ICP4 to promote viral transcription in herpes virus-infected human cells. Commun Biol, 4(1):510(2021) [pubmed]

submitter keyword: G-quadruplex, ICP4, HSV-1, proteins

Prof. Sara N. Richter
contact affiliation	Department of Molecular Medicine, University of Padua, via A. Gabelli 63, 35121 Padua, Italy
contact email	sara.richter@unipd.it
lab head
Ilaria Frasson
contact affiliation	Dept. Molecular Medicine, University of Padova, Italy
contact email	ilaria.frasson@unipd.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024743

PRIDE project URI

• PRIDE
  1. PXD024743
     1. Label: PRIDE project
     2. Name: Parallel G-quadruplexes recruit the HSV-1 transcription factor ICP4 to promote viral transcription in herpes virus-infected human cells