PXD024734

PXD024734 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of human visceral and subcutaneous adipocytes
Description	Adipose tissue (AT) distribution is an important determinant of cardiometabolic health. Increased visceral adiposity has been associated with a higher risk of obesity-related diseases. An important step in deciphering the molecular complexity of subcutaneous AT (SAT) and visceral AT (VAT) is to elucidate the molecular composition of the principal AT cell type, adipocytes. We present a comprehensive protein expression profile of abdominal subcutaneous (SA) and omental visceral adipocytes (VA). We isolated adipocytes from paired AT biopsies obtained during bariatric surgery of 19 morbidly obese women (BMI > 30 kg/m2) and performed state-of-the-art mass spectrometry to investigate their proteome profiles. We identified 3,686 proteins groups and found 1,140 differentially expressed proteins (adj. p-value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. In addition to providing a global protein profile of abdominal SA and omental VA, we also present the most differentially expressed pathways and processes distinguishing SA from VA. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and also to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondrial energy metabolism and translational or biosynthetic activity is higher in VA. We also performed prediction analyses of differentially expressed putative secreted proteins, which revealed a significantly higher number of potentially secreted proteins in SA compared to VA. Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity. Additionally, we predict possible protein targets among secreted proteins, which may be utilized for the further investigation of the role of different AT depots in obesity using peripheral blood.
HostingRepository	PRIDE
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2021-11-04_03:12:24.352.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Hruška
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-15 05:22:36	ID requested
⏵ 1	2021-11-04 03:12:25	announced

Hruska P, Kucera J, Pekar M, Hol, é, czy P, Mazur M, Buzga M, Kuruczova D, Lenart P, Fialova Kucerova J, Potesil D, Zdrahal Z, Bienertova-Vasku J, Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes. J Clin Endocrinol Metab, 107(3):755-775(2022) [pubmed]

submitter keyword: Human, adipocytes, LC-MSMS, obesity, adipose tissue, visceral adipose tissue, subcutaneous adipose tissue

Julie Dobrovolna
contact affiliation	Research Centre for Toxic Compounds in the Environment, Environmental Physiology, Faculty of Science, Masaryk University, Brno, Czech Republic
contact email	julie.dobrovolna@recetox.muni.cz
lab head
Pavel Hruška
contact affiliation	Central European Institute of Technology, Masaryk University, Brno, Czech Republic
contact email	394282@muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD024734

PRIDE project URI

• PRIDE
  1. PXD024734
     1. Label: PRIDE project
     2. Name: Comparative proteomics of human visceral and subcutaneous adipocytes