PXD024729

PXD024729 is an original dataset announced via ProteomeXchange.

Title	F1Fo-ATP synthase  subunit determines lethal infection in pathogenic fungi
Description	Fungal infections, especially for candidiasis and aspergillosis, claim an unacceptably high fatality. However, the determining mechanism that promote fungal lethal infections are still elusive. The energy ATP necessary for fungal cell growth and function is synthesized mainly through oxidative phosphorylation, whose key enzyme is F1Fo-ATP synthase. Nonetheless, it remians unknown how this enzyme affects fungal pathogenicity. Here we show that F1Fo-ATP synthase  subunit deletion completely abrogates C. albicans lethal infection rather than leading to remarkable intracellular ATP concentration and growth defect. Mechanically,  subunit deletion reduces PFK1 activity via interrupting PFK1 phosphorylation to trigger its conformational change, decreases downstream FBP level, blocks Ras1-dependent and -independent cAMP-PKA pathway, and curtails virulence factors. Based on these findings, we engineer a small molecule compound aimed at  subunit that effectively protects mice from succumbing to invasive candidiasis. In summary, our findings reveal that F1Fo-ATP synthase δ subunit determines the lethal infection of pathogenic fungi and represents a potential new therapeutic target.
HostingRepository	PRIDE
AnnounceDate	2021-09-16
AnnouncementXML	Submission_2021-09-15_21:34:29.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shuixiu Li
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-15 05:22:20	ID requested
⏵ 1	2021-09-15 21:34:29	announced

Dataset with its publication pending

submitter keyword:  subunit;lethal infection;pathogenic fungi

Hong Zhang
contact affiliation	Jinan University
contact email	tzhangh@jnu.edu.cn
lab head
Shuixiu Li
contact affiliation	Department of Dermatology, the First Affiliated Hospital of Jinan University, Guangzhou, Guangdong, China
contact email	lishuixiu@jnu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD024729

PRIDE project URI

• PRIDE
  1. PXD024729
     1. Label: PRIDE project
     2. Name: F1Fo-ATP synthase  subunit determines lethal infection in pathogenic fungi