PXD024708

PXD024708 is an original dataset announced via ProteomeXchange.

Title	How to make a ribosome from mixed-up ribosomal RNA fragments in Chlamydomonas reinhardtii mitochondria
Description	Mitochondria are essential components of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few, although essential, mitochondrial messenger RNAs still encoded by mitochondrial genomes. Here, we present the biochemical and structural characterization of the model green alga Chlamydomonas reinhardtii mitoribosome as well as the functional study of some of its Chlamydomonas specific components. We provide a single particle cryo-electron microscopy structure of Chlamydomonas mitoribosome and show how its core is composed by the assembly of 13 rRNA fragments encoded by separate non-contiguous gene pieces. While 5S rRNA was assumed to be absent from Chlamydomonas mitoribosome, a small rRNA representing a highly divergent 5S rRNA occurs in its central protuberance. In contrast, a gene piece that was believed to encode a fragment of rRNA encodes a small RNA that does not occur in the mitoribosome. Eleven novel proteins, mainly helical repeat proteins, including OPR, PPR and mTERF proteins occur in Chlamydomonas mitoribosome, revealing in particular the first structure of an OPR protein in complex with its RNA target. Reverse genetic studies reveal that the functions of some of the novel helical repeat proteins are required for mitoribosome biogenesis. The long studied p32 protein, whose function was related to several mitochondrial diseases in Human uniquely occurs as a constitutive ribosomal protein in the small subunit. Finally, cryo-electron tomography and biochemical studies show that Chlamydomonas mitoribosomes are exclusively attached to the mitochondrial inner membrane via two contact points mediated by Chlamydomonas specific novel proteins. One of them provides a direct interface between the exit of the peptide channel and the insertase Oxa1 for the insertion of nascent proteins in the mitochondrial inner membrane.
HostingRepository	PRIDE
AnnounceDate	2022-03-15
AnnouncementXML	Submission_2022-03-15_01:35:25.901.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD024708
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-12 08:28:46	ID requested
⏵ 1	2022-03-15 01:35:26	announced

Waltz F, Salinas-Gieg, é T, Englmeier R, Meichel H, Soufari H, Kuhn L, Pfeffer S, F, ö, rster F, Engel BD, Gieg, é P, Drouard L, Hashem Y, How to build a ribosome from RNA fragments in Chlamydomonas mitochondria. Nat Commun, 12(1):7176(2021) [pubmed]

submitter keyword: mitochondria

mitochondrial ribosome

mitoribosome

Chlamydomonas reinhardtii

cryo-EM

cryo-ET

fragmented ribosomal RNAs

Florent Waltz
contact affiliation	Institut Européen de Chimie et de Biologie, U 1212 Inserm, Université de Bordeaux, 2 rue R.Escarpit, F-33600, Pessac, France
contact email	waltz.florent@gmail.com
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024708
     1. Label: PRIDE project
     2. Name: How to make a ribosome from mixed-up ribosomal RNA fragments in Chlamydomonas reinhardtii mitochondria