PXD024691

PXD024691 is an original dataset announced via ProteomeXchange.

Title	Protein content of extracellular vesicles from human brain microvascular endothelial cells treated by paraquat
Description	Since the last decade, the field of extracellular vesicles (EVs) has increasingly grown. These “intracellular messengers” became interesting as biomarker source in the SNC research, because of their content, which may reflect the physiological state of their donor cells in a context of an external stimuli. However, still little is known about EVs released from cell types constituting the blood-brain barrier (BBB), especially on the human brain microvascular endothelial cells (HBMECs). The current study aimed to isolate and characterize EVs from HBMECs and to analyze the EVs proteome modulation after an external stimulate such as the Paraquat (PQ), a neurotoxicant. Size distribution, quantity and presence of EV markers were assessed. Identification and quantification of EVs proteins was conducted by data-independent acquisition mass-spectrometry (DIA-MS). Signature pathway of PQ-treated EVs were analyzed by gene ontology terms and pathway enrichment. Results present evidence that PQ-treated HBMECs and EVs are sharing proteins belonging to the ubiquinone metabolism and to the transcription HIF-1 targets pathways with a highly significant p-values, suggesting that those pathways may be a potential EVs signature of the PQ-induced toxicity in the BBB.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-16_23:19:26.916.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tatjana Vujic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 23:21:01	ID requested
⏵ 1	2022-02-16 23:19:27	announced

Vuji, ć T, Schvartz D, Iliuk A, Sanchez JC, Ubiquinone Metabolism and Transcription HIF-1 Targets Pathway Are Toxicity Signature Pathways Present in Extracellular Vesicles of Paraquat-Exposed Human Brain Microvascular Endothelial Cells. Int J Mol Sci, 22(10):(2021) [pubmed]

submitter keyword: extracellular vesicles, endothelia cells, paraquat, CNS, BBB, proteomics, DIA-MS, ubiquinone metabolism, HIF-1

Jean-Charles Sanchez
contact affiliation	Translational Biomarker Group, University of Geneva, Geneva, Switzerland
contact email	Jean-Charles.Sanchez@unige.ch
lab head
Tatjana Vujic
contact affiliation	Department of Medicine, University of Geneva, Geneva, Switzerland
contact email	tatjana.vujic@unige.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD024691
     1. Label: PRIDE project
     2. Name: Protein content of extracellular vesicles from human brain microvascular endothelial cells treated by paraquat