PXD024685

PXD024685 is an original dataset announced via ProteomeXchange.

Title	Proteomic analyses of non-preconditioned murine kidneys pre- and post-IRI in comparison to kidneys after preconditioning by hypoxia or caloric restriction
Description	12 – 14-week-old male C57Bl6N mice were divided into 3 treatment groups (n=5). First, non-preconditioned animals received ad libitum access to food under a daily 12 hour light and 12 hour dark rhythm with similar specific pathogen free (SPF) conditions in group-cages with up to 5 mice at the same time. Calorically restricted mice obtained 66% of the usual daily intake (3 g per day per mouse) 28 days before surgery as previously described (PMID: 30522767). For the hypoxic preconditioning mice were kept in a hypoxia chamber with a reduction of oxygen to 8.3% for 2 h, 4 h and 8 h on three following days prior to surgery based on the description of Bernaudin et al. (PMID: 11919510). On the day of Ischemia-Reperfusion Injury (IRI) all mice were anesthetized by ketamin (Zoetis (Berlin, Germany))/xylazine (Bayer (Leverkusen, Germany)) and afterwards all mice received a midline abdominal incision as previously described (PMID: 27557097). After ligation of the right kidney vessels right nephrectomy was performed and the kidneys were cut into halves. One half was frozen in liquid nitrogen for proteomic analyses. Then, the left renal vessels were clamped for 40 minutes followed by a subsequent removal of the clamp for reperfusion. The abdominal incision was closed. Subsequently, the mice were placed into single cages. 4 h after reperfusion and following another anesthesia the left kidneys were taken out. Again, the kidneys were cut into two halves and one of it was preserved in liquid nitrogen for proteomic analyses.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:55:36.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Hoehne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-03-11 22:56:42	ID requested
1	2023-02-13 00:53:55	announced
⏵ 2	2023-11-14 07:55:40	announced	2023-11-14: Updated project metadata.

Sp, ä, th MR, Hoyer-Allo KJR, Seufert L, H, ö, hne M, Lucas C, Bock T, Isermann L, Brodesser S, Lackmann JW, Kiefer K, Koehler FC, Bohl K, Ignarski M, Schiller P, Johnsen M, Kubacki T, Grundmann F, Benzing T, Trifunovic A, Kr, ü, ger M, Schermer B, Burst V, M, ü, ller RU, Organ Protection by Caloric Restriction Depends on Activation of the De Novo NAD+ Synthesis Pathway. J Am Soc Nephrol, 34(5):772-792(2023) [pubmed]

submitter keyword: hypoxia, caloric restriction,Ischemia-Reperfusion Injury, preconditioning

Roman-Ulrich Müller
contact affiliation	1) Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany 2) CECAD, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany
contact email	roman-ulrich.mueller@uk-koeln.de
lab head
Martin Hoehne
contact affiliation	1) Department II of Internal Medicine and Center for Molecular Medicine Cologne, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany 2) CECAD, University of Cologne, Faculty of Medicine and University Hospital Cologne, Cologne, Germany
contact email	martin.hoehne@uk-koeln.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/02/PXD024685

PRIDE project URI

• PRIDE
  1. PXD024685
     1. Label: PRIDE project
     2. Name: Proteomic analyses of non-preconditioned murine kidneys pre- and post-IRI in comparison to kidneys after preconditioning by hypoxia or caloric restriction